| Summary: | 碩士 === 國立陽明大學 === 解剖學及細胞生物學研究所 === 101 === Gastric cancer is the fourth most common cancer and the second leading cause of cancer-related death in the world. Many factors can cause gastric cancer, just like infection of Helicobacter pylori, improper habit of life and diet, even an inherent cause. The major therapies of gastric cancer are gastrostomy, chemotherapy and radiation treatment, but the prognosis for advanced patients is still poor. In this study, the microRNA-425 (miR-425)-expressing adenoviral system was established. SC-M1 gastric cancer cells infected with miR-425-expressing adenovirus (Ad-miR-425) increased two fold of miR-425 as compared with those infected with green fluorescent protein (GFP)-expressing adenovirus (Ad-GFP). Endogenous miR-425 expressed differentially in seven kinds of gastric cancer cell lines. In the biological function, we found that overexpression of miR-425 inhibited cell migration, invasion and colony forming abilities to repress progression of gastric cancer. The results of MTT assay and cell growth demonstrated that miR-425 decreased cell viability and cell growth to suppress tumor cell growth. The results also found miR-425 decreased sphere forming ability of gastric cancer stem cells to inhibit cancer progression. In this study, data showed that miR-425 decreased Jagged1 expression, whereas increased Notch 3 receptor expression. Previously, Notch 1 receptor promoted progression of gastric cancer via influencing expression of downstream genes. The results of this study using real-time PCR assay demonstrated that overexpression of Notch 1 receptor suppressed miR-425 expression. Taken together, miR-425 plays an important role in inhibiting gastric cancer progression. In the future, miR-425 may be a novel therapeutic target and biomarker for the therapy of gastric cancer.
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