Evaluations of chitosan membrane combined with electrospinning nanofiber as skin substitutes

• Main Authors: Shu-Jing Li, 李淑靖
• Other Authors: Kuo-Yu Chen
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/11008568814700708689

碩士 === 國立雲林科技大學 === 化學工程與材料工程系碩士班 === 101 === A bilayer membrane composed of chitosan and electrospun nanofibers was prepared as a novel skin substitute. Chitosan is widely applied for wound dressings due to good biocompatibility and antibacterial property. However, cells can not attach on the surface of chitosan membrane in a short time. This study prepared various ratios of gelatin and fish collagen peptide nanofibers via electrospinning to improve cells attachment. The nanofibers consisted of different weight ratios of gelatin to fish collagen peptides (10:0 (GF100), 9:1 (GF91) and 8:2 (GF82)). The lithospermi radix was incorporated into the nanofibers to improve the antibacterial property of membranes. The nanofibers were then crosslinked with glutaraldehyde vapor to improve their structural stability in water. The compositions, surface morphology, swelling ratio, degradation rate, drug release rate and cell compatibility of materials were evaluated. The result shows that N=C bond was formed on the surface of chitosan membrane after surface treatment with glutaraldehyde vapor. The nanofibers were then fixed onto the surface of chitosan membrane via the reaction between N=C bond and nanofibers. The chitosan membrane had a high swelling ratio of 1909 wt% in water due to its porous structure. After incubation in water for 28 days, the degradation rate of chitosan membrane was about 10.5 wt%. Scanning electron microscopy (SEM) pictures revealed that the diameter of nanofibers decreased with the increase of voltage. The diameters of GF100, GF91 and GF82 nanofibers were 279.20 ± 20.00, 182.86 ± 48.67 and 206.66 ± 50.34 nm,respectively. GF82 nanofibers have a higher degradation rate than GF91 and GF100 nanofibers. Moreover, GF82 nanofibers degraded completely within 21 days. Lithospermi radix was totally released from GF82 nanofibers within 1.5 hours. However, lithospermi radix was released slowly from GF91 nanofibers. Additionally, lithospermi radix was not able to release from GF100 nanofibers. Cytotoxicity analysis demonstratrd that gelatin, fish collagen peptides and chitosan released from the membranes were not toxic to human fibroblasts. SEM pictures showed that nanofibers could enhance cells attachment. Moreover, these cells exhibited flattened morphology. These results indicates that the bilayer membrane prepared in this study has a good potential as a skin substitute.