Bioethanol production from rice straw by an innovative bioreactor

• Main Authors: Yu-Hsuan Liu, 劉又瑄
• Other Authors: Yu-Hong Wei
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/69485751830070412326

碩士 === 元智大學 === 生物科技與工程研究所 === 101 === Bioethanol production was still currently using the traditional two-stage fermentation process, the first stage of the hydrolysis of cellulosic saccharification and fermentation, and the second stage will be the use of alcoholic fermentation bacteria sugars into alcohol. Based on simplified procedures and reduce production cost considerations, Simultaneous saccharification and co-fermentation (SSCF), is currently developing lignocellulose bioethanol important technologies. In this study, we have developed an innovative bioreactor that simultaneously metabolism pentose and hexose fermentation process for bioethanol production. Firstly, Aspergillus niger and Trichoderma reesei are co-immobilized on PU (polyurethane) carrier, then Saccharomyces cerevisiae alginate beads were added into this bioreactor to co-culture overcome the problem of simultaneous saccharification and bioethanol fermentation by using rice straw medium. Lignocellulose was converted into reducing sugars by using saccharification enzymes from co-cultivated Aspergillus niger and Trichoderma reesei and reducing sugars as nutrients for Saccharomyces cerevisiae to produce bioethanol in an immobilization system. Experimental results prove that the immobilized Saccharomyces cerevisiae could be able to produce bioethanol is 1.3-fold than without immobilized strain. It’s also showed that the circulation rice straw medium increased ethanol production. We also show that the rice straw concentration is increased could produce higher concentration of bioethanol. Furthermore, we can obtain the highest bioethanol production (1.057 g/L) after the co-cultivation of 72 hr..