| Summary: | 碩士 === 國立中正大學 === 化學暨生物化學研究所 === 102 === Ionic liquids (ILs) have many unique properties including high thermal stability, high conductivity, almost negligible vapor pressure, and therefore are considered as green solvents. They also exhibit tunable physicochemical properties and can be rationally designed for desired applications. So they are regarded as designer solvents. ILs have multiple modes of molecular interactions, including hydrogen bond, ion-exchange, hydrophobic, and π-π interactions. Therefore, they have been employed as the chromatographic stationary phases to separate different types of compounds in a single column in chromatography.
Carbon dioxide supercritical fluid, polarity similar to that of n-hexane, chromatography (SFC) is usually perceived as a potential alternative of normal-phase liquid chromatography. This is due to the mobile phase is a non-polar medium. In addition, carbon dioxide supercritical fluid is non-toxic and can reduce the use of organic solvents. Therefore, carbon dioxide SFC is considered as a green separation method. SFC columns normally adapted from traditional liquid chromatography column so far. There are very few columns that are special designed for SFC. Special designed stationary phase for SFC is desired to enhance the performance of this green separation method.
In this study, we explored the structure of benzimidazolium ionic liquid to design a series of stationary phases for SFC. The benzimidazolium ionic liquids were chemically bounded onto the silica gel to prepare the stationary phases of SFC column. Then the LSER model was employed to characterize the molecular interactions of the columns and related the molecular interactions to their structures. In addition, the benzimidazolium stationary phases were also applied to the separation and analysis of drugs to test their performance in real samples. The recoveries are in the range of 99-109%. The reproducibilities of different batches of the column are less than 10%. The preliminary results indicate that these benzimidazolium ionic liquid columns have high potential for SFC.
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