Discussion the optimal liquid Antrodia camphorata apply whitening effect analysis

• Main Authors: Sheng-Yen Chuang, 莊笙彥
• Other Authors: Jong-Tar Kuo
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/34495148651356929677

碩士 === 中華科技大學 === 健康科技研究所 === 102 === Many studies have confirmed. The tyrosinase is a key enzyme that melanoma cell produce melanin. Therefore, in assessing the whitening cosmetics and development process. Find out which tyrosinase inhibitors are the key of whitening. This study will use different formulations to liquid culture of the Antrodia camphorata. Collection the mycelium on different time. Extract the mycelium on a different solution. Take some extract solution sample to test tyrosinase inhibition assay and use the HPLC to analysis ingredient and MTT assay to cytotoxicity. We hope in the experiment can find inhibit melanin production of active ingredients. To make active ingredients have some whitening effect. The extraction solvent has methanol, distilled water and ethyl acetate. The results show. The Antrodia camphorata cultured in different liquid medium in 10 days, 15 days and 20 days. We analysis the dry weight by statistical. The P0.5 have a significant difference in 15 days and 20 days. The rest of the conditions are not significantly different. In the tyrosinase inhibition test. The best tyrosinase inhibition effect is the ethyl acetate extract solution. The highest inhibition rate have 25.8%. In HPLC chromatogram. The liquid Antrodia camphorata extract peaks mostly concentrated on the 30 to 40 minutes by methanol and water extract. But the ethyl acetate extract sample is ending on 200 min. Although peaks height is less than the methanol and water extract. We can see the ethyl acetate extractbeing more composition than methanol and water extract. Diverted the peak A, B, C, D and E from the sample, respectively tyrosine test. The test shows that the peak C and E are better than the other. In cell test. Diverted the peak A, B, C, D and E from sample, used the human fibroblast proliferation and human melanoma tumor cell inhibition test. The results can be found, peak B and peak C Effect is better by the human fibroblast proliferation test. The peak A and B have the inhibition effect of human melanoma tumor cell inhibition test. The peak C, D, E may can promote growth of the cell.