| Summary: | 博士 === 中國醫藥大學 === 中醫學系博士班 === 102 === The character of wind is quick and easy changes, and wind causes diseases are the most common, such as common cold. The blood returns to the liver during the sleepy. Wind is belong to liver system, thus, liver blood deficiency can produced ascendant hyperactivity of liver yang, or liver fire flaming upward resulting in liver wind. Therefore, wind divides into internal wind such as liver wind, and external wind such as common cold. Liver wind is resulting from imbalance between yin and yang in liver system, i.e. imbalance between excitation and inhibition, such as epileptic seizure, neuropathic pain and migraine etc., and these are identical paroxysmal disorders in modern medicine. Therefore, the purpose of the present study was to investigate therapeutic effect and mechanism of Chinese medicine treating paroxysmal disorders focus in epileptic seizure and neuropathic pain. The study divided into experimental one: using Uncaris rhynchylla (UR) and rhynchophylline (RP) pretreatment for three days, and then induced epileptic seizure by kainic acid (KA, 12 mg/kg) i.p. in the Sprague-Dawley (SD) rats. The rat was sacrificed and the brain was removed 3 h after KA administration. Cytokines including interleukin(IL)-1β, IL-6 and tumor necrosis factor-α, (TNF-α) and mitogen activator protein kinase (MAPK) signal pathway and nuclear factor-κB (NF-κB )were observed in the cerebral cortex and hippocampal brain tissues; experimental two: the chronic constriction injury (CCI)-induced neuropathic pain animal model was established in the SD rats by using 4-0 chromic gut ligated loosely in the right sciatic nerve, and then investigated the therapeutic effect and mechanism of electroacupuncture (EA) on neuropathic pain including behavior and transient receptor potential vanilloid type 4 (TRPV4) and TRPV1. The results: both UR and RP pretreatment can reduce epileptic seizures and the expression of c-Jun-N-Terminal Kinase phosphorylation (JNKp) of MAPK; both 2Hz and 15Hz EA could reduce withdraw latency difference of left-right hind-limbs in the radiant heat test, and could reduce lift legs counts in the 4℃ cold plate test, and also could increase gram number in the von Frey test. In addition, both 2Hz and 15Hz EA could reduce the expression of TRPPV4 in the cerebral cortex.
In conclusion, both UR and RP have anticonvulsive effect, and anticonvulsive effect has relationship to JNKp of MAPK signal pathways in acute seizure stage; both 2 Hz and 15 Hz EA can reduce CCI-induced neuropathic pain, the this effect of reducing neuropathic pain is mediated via multiple pathways, and suggesting cerebral TRPV4 is involved.
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