Effects of purple sweet potato leaf extract (PSPLE) on 3T3-L1 adipocyte differentiation

• Main Authors: Yu-Jie Wang, 王禹傑
• Other Authors: Shou-Lun Lee
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/kqr354

碩士 === 中國醫藥大學 === 生物科技學系碩士班 === 102 === Obesity is a health hazard which is closely associated with metabolic syndromes which are manifested by many symptoms like elevated, glucose, cholesterol, triacylglycerol, increases in blood pressure, risk of cardiovascular disease, and heightened chances of being diagnosed as type II diabetes. Adipocytes play a critical role in regulating lipid metabolism and energy balance and are associated with adipose tissue mass and obesity. Purple sweet potato leaves (PSPL) contain a high content of anti-oxidants which have been shown to exhibit a number of biological activities. The aim of this study was to investigate whether purple sweet potato leaf extract (PSPLE) could affect differentiation of 3T3-L1 pre-adipocytes. Our data showed that PSPLE significantly decreased the proliferation of as determined by MTT assay, but didn’t lower cell viability by Trypan blue exclusion test. These results also indicated that no cytotoxicity on 3T3-L1 preadipocytes was observed after treatment with PSPLE. In addition, our results demonstrated that PSPLE induced anti-adipogenesis during the differentiation of 3T3-L1 preadipocytes. PSPLE may decrease the lipid content of differentiated 3T3-L1 cells via induction of cell apoptosis. Taken together, PSPLE may be beneficial to anti-obesity effect and preventing metabolic syndrome.