The study of zinc oxide nanoparticles and cigarette-smoking extracts impair nontypeable Haemophilus influenzae clearance by macrophages

• Main Authors: Chin-Yu Liao, 廖謹瑜
• Other Authors: Chih-Ho Lai
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/7552jw

碩士 === 中國醫藥大學 === 基礎醫學研究所碩士班 === 102 === Nontypeable Haemophilus influenzae [1] is a Gram negative coccobacillus, which belongs to the normal flora of the human respiratory tract. When host immune mechanisms were defective, NTHi could invade into the lower respiratory tract, cause acute exacerbation of bronchial airway disease or chronic colonization. Previous study showed that nanomaterials (e.g., silver, iron, zinc oxide, titanium dioxide) have a variety of applications. Nanoparticles can deliver to the bloodstream or lymph nodes and then transfer to other organs, and easily penetrate the cytoplasmic membrane followed by getting into the nucleus. NTHi is the pathogen which was isolated from lower respiratory tract and secretions from chronic respiratory diseases such as chronic obstructive pulmonary disease (COPD) and chronic bronchitis, and more than 40% to 60% of those patients became acute exacerbations of COPD (AE-COPD). In vitro experiment showed that treatment of RAW264.7 cells with ZnO-nanoparticles (ZnO-NPs) can decrease macrophage clearance of NTHi, and lead to downregulation of NTHi-activated expression of inducible nitric oxide (NO) and translocation of active NF-κB into the nucleus. Additionally, in the study of cigarette smoking extracts [2], we found that treatment of RAW264.7 cells with 10% CSE and exposure to NTHi, the mRNA and protein expression levels of CCL-2, CXCL-2, CXCL-10 were decreased. Our results demonstrate that the initial performance of innate sensing under the exposure of CSE and ZnO-NPs which may affect the clearance of NTHi and therefore lead to disease become more severity.