| Summary: | 碩士 === 嘉南藥理大學 === 生物科技系 === 102 === A novel multiple membrane blood-feeding system for mosquitoes has been developed for the study and routine maintenance of Aedes aegypti L. for mosquitoes that normally require vertebrate blood meal to produce eggs. This blood-feeding system uses cattle collagen sausage casing membrane to facilitate feeding. The efficiency of this blood-feeding system was compared to a live mice blood source. We observed that Ae. aegypti that fed on pig whole blood had 89.7% (w/o ATP) and 90.7% (w/ ATP) blood feeding rates, which were not significantly different from the mice-fed ones (98.0%). The feeding rates did not differ between sausage casing membrane and Parafilm-M®. The survival rate, fecundity, pupation, and pupal emergence rates of Aedes females fed on pig whole blood were not significantly different from the mice-fed ones. The artificial blood feeder can be applied to replace live animals as blood sources. An artificial blood meal has been developed for feeding Aedes aegypti (L.), a mosquito that normally requires a vertebrate blood meal for engorgement. The artificial blood meal is a simple mixture with salt, albumin, dextrose, and adenosine triphosphate added to induce engorging. The feeding rates of Aedes aegypti on the artificial blood meal were not significantly different from those on pig blood, but the feeding rates were significantly higher than on CPDA-1 solutions. Because most assays currently rely on human subjects or vertebrate whole blood, an efficient and safe in vitro assay using a artificial blood meal and a multiple-membrane blood-feeding device could facilitate the development of a significantly simplified screening system for possible novel repellents. The dose-dependent biting inhibition rates were analyzed by probit analysis. The ID50 (the dose producing 50% inhibition of mosquito feeding) values of DEET, citronella, carvacrol, geraniol, eugenol and thymol were 1.62, 14.40, 22.51, 23.29, 23.83 and 68.05 μg/cm2, respectively.
Keywords: Aedes agypti, blood feeder, in vitro repellent bioassay, essential oils。
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