The anti-diabetic and anti-inflammatory effects of baicalin in the C2C12 skeletal muscle cells

• Main Authors: Hsiao-Tzu Kuo, 郭小慈
• Other Authors: 吳淑靜
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/n4u8te

碩士 === 嘉南藥理大學 === 保健營養系 === 102 === Baicalin is a flavonoid that possess antioxidant, anti-viral, anti-cancer and other physiological activities. The aims of this study were (1) to study the anti-diabetic effect of baicalin in the murine C2C12 skeletal muscle cells, and (2) to examine the anti-inflammatory activity of baicalin in palmitic acid (PA)-induced cells. The results from the study showed protection effects at all concentrations of baicalin tested. From 25 to 50 μM baicalin significantly up-regulated of the glucose absorption. The results from western blot revealed that baicalin effectively up-regulated the expression of PI3K, p-Akt, p-IRS-1 and GLUT4 proteins, with less obvious effect on p-ERK. Furthermore, baicalin did not affect the expression of Akt, p38, p-p38, JNK, p-JNK, and ERK proteins. Results on baicalin is effect on palmitic acid-stimulated cell function revealed that cell viability after palmitic acid stimulation reduced to 70% while baicalin protected the cells to 90%. Baicalin remarkly suppressed the expression of IL-6 and IL-10 in PA-stimulated cells. The inhibitory effect on TNF-α was not significant. These results suggest that baicalin may play a potentral role in the anti-diabetic and anti-inflammatory effects through glucose up-take, insulin signaling pathways, and inhibiting the production of inflammatory cytokines.