The Establishment of Novel Eicrobiological Sampling and Quantitative System in Recreation Water

• Main Authors: Wen Rong Liu, 劉文榮
• Other Authors: Chi-Chuan Kan
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/97016409188603963812

碩士 === 嘉南藥理大學 === 觀光事業管理系 === 102 === In recent years, national income increases and people's living standards have improved so that more and more leisure activities are emphasized, such as hot spring and Spa are the prevalence of leisure activity for the past few years. Therefore, hygiene and safety in water quality issue is important. However, the more number of people using bathing pool the more consumption of residual chlorine, then the general water quality cannot be notified immediately since the number of microorganisms raise in water. As a result, a routine sampling and analysis is required to maintain good water quality. However, the present standard method is time-consuming and spends more cost and drug on it, yet it cannot provide the general condition in water quality immediately, which makes it becomes a serious problem to maintain water in a good condition. Consequently, this study uses the metallic membrane filtration and Gram stain with compact USB Microscope to establish a simple filter microscopic examination system, which calculates the average area of microorganisms via utilizing USB Microscope and computer software and compare it with EPA (Environmental Protection Administration) regulations, announcement for standard methods of total plate count (TPC)-smear, then discuss the relevance of analysis method and image analysis. The result indicates the correlation R2 value is 0.8975 between smear and simple filter microscopic examination method, which means both ways have a positive linearity. Moreover, it refers that the amount of microorganism can be detected in both TPC smear and simple filter microscopic examination method when the area of microorganism exceeds 0.048% and 0.032% respectively. Therefore, it demonstrates that simple filter microscopic examination one is much more sensitive than the smear one.