Oleic Acid and Glucose Induced Vascular Smooth Muscle Cell and Breast Cancer Cell Proliferation and Migration and its Mechanisms

• Main Authors: Yi-Sun Yang, 楊宜瑱
• Other Authors: 黃建寧
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/59878347273403406670

博士 === 中山醫學大學 === 醫學研究所 === 102 === To investigate the role of glucose and oleic acid in the expression of connective tissue growth factor (CTGF) and fatty acid synthase (FASN) in human vascular smooth muscle cells (VSMCs) and breast cancer cells (MDA-MB-231 cell) and its possible mechanisms. VSMCs and MDA-MB-231 cell were incubated in the presence of glucose, oleic acid and both at the concentrations ranging from 25 to 30 mM and oleic acid of 200 uM, and Western blotting was used to measure the protein expressions of CTGF and FASN. Oleic acid could induce both migration and proliferation of VSMCs and MDA-MN-231 cells. Oleic acid induced VSMCs proliferation is mediated by CTGF and FASN, whereas migration is mediated by CTGF, by activating focal adhesion kinase. Glucose induced MDA-MB-231 cells proliferation is mediated by FASN, whereas oleic acid induced proliferation is mediated by CTGF. Both glucose and oleic acid increased the vimentin expression, which could be inhibited by metformin. Glucose and oleic acid could induce both migration and proliferation of VSMCs and MDA-MB-231 cells, mediated by CTGF and FASN. Metformin could play a role of inhibit breast cells invasion.