| Summary: | 碩士 === 中原大學 === 生物醫學工程研究所 === 102 === Recently, the unique optical properties of quantum dot gradually replace the traditional organic fluorescent dyes and fluorescent proteins in the field of biomedical technology. It has become important tools in related biomedical areas, such as cell targeting, cell tracking. But the material for the synthesis of traditional quantum dot contains heavy metal, such as Cd2+、Pb2+, which will cause serious toxicity for both biological and environmental pollution. Scientists have investigated the alternative materials to replace heavy metal ions and thereby increase its potential in biomedical applications. This study focused on engineering hydrophobic InP/ZnS quantum dots and on the use of octenyl succinic anhydride (OSA) toward bioconjugation. Using a simple, fast microemulsion method not only can modify the hydrophobic InP/ZnS Quantum dots to be hydrophilic ones but also transfer versatile hydrophobic nanoparticles to aqueous phase successfully. However, the buffer layer structure conposed by the octenyl succinic anhydride was unsteady, it was prone to result in disintegration in diluted condition. Then we further showed that using polyethylene glycol monomethyl ether as the surface modifier can increase the stability of InP/ZnS hydrophilic structure. Subsequently, using DSPE-PEG-X (X: -amine, -carboxyl, -methoxy) can further functionalized the surface which can be verified by gel electrophoresis. In this study, we found that the use of octenyl succinic anhydride can offer superior hydrophilic modification of InP/ZnS quantum dots and that the use of polyethylene glycol monomethyl ether can increase stability of buffer layer to improve the stability after phase transfer. We envision the coating method will expand the nanomaterials applying in biomecial research.
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