Effects of Environmental and Metabolism Control Strategy on Mycelial Biomass and Bioactive Ingredients Production by the Submerged Culture of Xylaria nigripes

• Main Authors: PAN,HOU-LIANG, 潘厚良
• Other Authors: XU,TAI-HAO
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/01811148102415094370

碩士 === 大葉大學 === 生物產業科技學系 === 102 === 　　Xylaria nigripes, named Wū-Línɡ-Shēn in Chinese, belongs to the division of Ascomycotina, the family of Xylariaceae, and down to genus of Xylaria. X. nigripes is a precious medicinal fungus that grows on top of the abandoned termite nests underground. Based on the Chinese pharmacopeia, Wū-Línɡ-Shēn has several biological functions such as tranquilizer, enhancing physical energy, maintaining renal function, dehumidification effect in spleen, treating depression, neurasthenia, and insomnia and palpitations embolism. 　　Due to its difficulty to plant and less productive quantity, there is a little of information about Wū-Línɡ-Shēn. Researchers studying on liquid state-fermented of Wū-Línɡ-Shēn is few. The major purpose of this study is to test the environmental factors for Wū-Línɡ-Shēn to culture under liquid state fermentation. Then, the production was scaled up to a 20L fermenter and further analyzed the contents of mycelium biomass and bioactive ingredients. 　　The experiments were conducted based on factors, including temperature, pH, two-phase selectivity and concentration, volume of inoculation, ventilation, and cultivation time. The results indicated the optimized condition for optimal production were 5 day’s growing, temperature at 25 degree Celsius, pH-state at 4, and 2vvm ventilation with 0.1% soybean oil, 10% inoculation. The amount of biomass, extracellular polysaccharide, intracellular polysaccharide, total polyphenol, crude triterpenoid, and adenosine was 2.43mg/mL、1.75mg/mL、46.42mg/g、4.81mg/g、37.36mg/g and 4.319mg/g, respectively. 　　In conclusion, compared to standardized procedure, we except the extracellular polysaccharide, the yield of mycelium and other bioactive ingredients would increase up to four times at liquid state fermentation of Wū-Línɡ-Shēn in 20L fermenter.