The Studies of Haematococcus pluvialis Cultivation for Astaxanthin Production

• Main Authors: CHUANG KAI CHUAN, 莊凱荃
• Other Authors: 張振昌
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/t7uk8n

碩士 === 逢甲大學 === 化學工程學系 === 102 === Haematococcus pluvialis contain with abundant natural astaxanthin, which is full of antioxidant capacity that can quench free radicals, improve immune system, and prevent aging and cancer. Up to now, natural astaxanthin has been discovered as a most powerful antioxidant in natural resources. In this study, it’s divided into two steps to cultivate Haematococcus pluvialis. Before the first step, we found that replacing fresh medium regularly can nurture Haematococcus pluvialis effectively. In the first step of culture, use Taiguchi method to search for the optimal experimental condition for Haematococcus pluvialis production. The result shows that the optimum growth rate is 0.262 d-1, with the medium (JM) in N:C:P=1:2:5, irradiation of red light LED at illumination of 1600 lux, culturing for 10 days. In the second step of culture, it shows that the color in Haematococcus pluvialis will be accelerated from green to red, and get the concentration of Astaxanthin about 3.2 mg L-1, with the medium (JM), which is without main Nitrogen, irradiation of blue light LED at illumination of 8000 lux. Using vertical tubular photobioreactor to cultive Haematococcus pluvialis for 7 days in un-circulated condition, the concentration of algae reached 50×104 cell mL-1. As a result, the growth rate of algae was 0.291 d-1.