| Summary: | 碩士 === 逢甲大學 === 自動控制工程學系 === 102 === In this study, we developed the novel technique with fast and high efficiency detection on single cell using physical and chemical methods. In physical method: measurements of optical tweezers forces on Multiple-Resistant Staphylococcus Aureus (MRSA) can be used to detect the bacteria drug resistance by their wall thickness. Our results found the optical forces were directly proportional to wall thickness of bacteria. Because this resistance is associated with the increased growth and reduced autolysis of bacteria cell wall, our experiment has been successfully applied to classify different groups of MRSA, including VSSA (NCTC 10442), VISA (Mu 50), and heto-VISA (Mu 3). In chemical method: species-specific PCR can directly detect the gram-positive bacteria. A 3D pad of GFP-AcmA’proteins was made by two-photon induced laser photochemistry to bind gram-positive bacteria and directly observation by microscopy. For fast and high efficiency detection, we propose a 3D mesh structure in the microfluidic chip. To verify this idea, the flow and particle passing through 3D mesh in microfluidic channel was simulated using mathematical model with different elevation angle of 3D mesh structure, then a 3D Ormocomp mesh with the optimized design by simulation result was fabricated in the microfluidic chip to explore the flow and particle distribution in reality.
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