Applicability Of Photodynamic Treatment To Inactivate White Spot Syndrome Virus

• Main Authors: Lin,Chun-Wei, 林君威
• Other Authors: Li, Si-Shen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/04178525906923928178

碩士 === 輔仁大學 === 生命科學系碩士班 === 102 === White Spot Syndrome Virus (WSSV) is a serious viral disease to the shrimp culture industry. This study aims to create a powerful and energy-efficient method to inactive WSSV: Photodynamic treatment (PDT), to control the spread out of WSSV in shrimp’s culture pond. The WSSV was produced by Procambarus clarkii after muscle injection for 7 days .The energy-efficient LED and photosensitizing dyes were used in PDT for WSSV inactive. One hundred folds LD50 of WSSV were treated with PDT and injected into Litopenaeus vannamei’ muscle. The data showed the mortality rate were down from 100 to 33 % if the PDT is carry out with 160 mW /cm2 power consumed, 80 sec irradiation and 0.1 mM photosensitizing dyes. Excitingly, the mortality rate will down to zero only with the irradiate duration were increased to 100 sec. If the power consumed up to 225 mW /cm2, the mortality rate were down from 100 to 3 % with 20 sec irradiation. And the mortality rate also down to zero only if the irradiation time were up to 40 sec. Ten days after infection, the analysis of PCR for shrimp’s blood show that all of survival organisms don’t have any WSSV. On the other hands, if we take the healthy L. vannamei to carry out the PDT which could fully inactive WSSV (WPDT), results showed that the WPDT has not effect on the survival of shrimp during experiments. Under these results, we confirmed that the PDT has highly application potential to control the spread out of WSSV.