The Dynamic Influence of Photodynamic Theatment to the Association Between Nannochloropsis Oculata and Bacteria.

• Main Authors: Lin, Lin-Yen, 林伶燕
• Other Authors: Li Si-Shen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/62441935962615913044

碩士 === 輔仁大學 === 生命科學系碩士班 === 102 === Nannochloropsis oculata can be considered as a potential biomass for aquaculture, greenhouse gas reduction, healthy food and bio-diesel production. However bacteria, fungi and viruses often caused decreased productivity in large-scale cultivation. The first aim of this survey is using Photodynamic treatment (PDT) techniques to inactive the completely organisms that may interfere the productivity of N. oculata. LED, a cheaply and energy efficiently light source were used in these experiment. Several conditions of PDT, like power intensities, radiation time and light-sensitive agent were tested. Results showed that PDT can remove over the 93.85 % (±1 %) of the microbes in the algae broth under 0.1 mM light-sensitive agent, 700 mW/cm2 LED light source power and one minute illumination time. PDT can improve production efficiency of N.oculata for 33.6 % (after 27 days) under 0.1 mM light-sensitive agent, 225 mW/cm2 LED light source power and one minute illumination time. Howerve, the PDT is the non-specific sterilizations, so some probiotics may also be removed in these processes. Therefore, a second object of this study is to identify the probiotics organisms which were remove by PDT and define the effects of N. oculata. We find three microbial 4c、4e and 5b can improve the production rate of N.oculata for 23, 49 and 33 %, respectively.