Analysis of polyphenols in Lobelia chinensis Lour. and Chenopodium formosanum Koidz. by HPLC

• Main Authors: Po-Wen Huang, 黃柏文
• Other Authors: Jenn-Yuan Sheu
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/93500023989553643929

碩士 === 中華醫事科技大學 === 醫學檢驗生物技術系碩士班 === 102 === Phenolic compounds are the popular studies in the present day , cause of antioxidants. In this day, many people are using phenolic compounds to prevent disease from Plant extract. More and more people eat plant extract products , because it is not only can antiseptic or anti-virus but also inhibit LDL oxidation and prevention cardiovascular disease. High performance liquid chromatography with UV separation of these phenolic compounds are possible on C-18 column, using 0.1% formic acid and acetonitrile with 0.1% formic acid as mobile phase. Flow-rate, detection wavelength and temperature were adjusted to 1.0 mL/min, 285nm and room temperatur, respectively. Precision of measurements within 0.05 ~ 14.33 % were achieved. Accuracy of this sample analysis method was assessed by performing spikes and recovery experiments. Recoveries of added standard solution were found to be within 91 ~ 112.03 %, demonstrating good reliability of results. In this study ,we analyzed three plants as follows: fresh and drier’s Lobelia chinensis Lour. and Chenopodium formosanum Koidz.. In the future, the unknown peaks would be detected by gas chromatography – mass spectrometry(GC-MS).