| Summary: | 碩士 === 高雄醫學大學 === 香粧品學系碩士班 === 102 === L-Carnitine (3-Hydroxy-4-trimethylammoniobutanoic acid), butyrobetaine (4-trimethylammoniobutanoic acid), acetyl-L-carnitine (3-acetyloxy-4-trimethylammoniobutanoic acid) are three quaternary ammonium compounds which exist naturally in the human body. Due to L-carnitine is an important cofactor for fatty acid oxidation in the mitochondria, it is used as a drug for patients with fatty acid metabolism deficiency and also as an adjuvant for losing weight. Butyrobetaine is a precursor of endogenous L-carnitine, which is relative to biosynthesis of L-carnitine. Acetyl-L-carnitine, the derivative of L-carnitine, possesses neuroprotection and the capacity to decrease the production of free radicals.
For application of cosmetic, pharmaceuticals and food, the linear response was ranged from 10-2000 nM for L-carnitine and acetyl-L-carnitine with good correlation coefficient (r2>0.998) in standard solution. The detection limits of two analytes were 4.5 fmol. Because L-carnitine, betyrobetaine, and acetyl-L-carnitine are endogenous in human, we used standard addition method as a quantitative method and applied this method to human plasma successfully. The concentration of L-carnitine is in the range from 43.25 to 47.67 μM in health volunteers. In human plasma, butyrobetaine and acetyl-L-carnitine are in the range from 1.08 to 1.27 μM and 2.79 to 6.65 μM, respectively. The linearity (r2>0.996) and reproducibility of standard addition method were good enough for quantitation. The results showed that iDLLME combined the proposed method were successfully developed for analysis of water-soluble compounds, such as L-carnitine, butyrobetaine, and acetyl-L-carnitine.
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