| Summary: | 碩士 === 國立中興大學 === 化學系所 === 102 === In this study, a sensitive analytical method was developed for determination of the trace ultraviolet filters (UV filters) and metabolites in water and urine samples. Polypyrrole/multi-walled carbon nanotube composites (PPy/MWCNT) was successfully synthesized, and it can be applied as sorbents for extraction combined with gas chromatography- tandem mass spectrometry. In optimum conditions, 500 μLwater at pH4 or original urine samples containing 13 ultraviolet filters was added into 1.5 mL eppendorf. Then, 200 μL PPy/MWCNT solution was added into the sample and the mixture was sonicated (560W) for 30 seconds for extraction. After centrifugation, the supernatant was removed. 125 μL ethyl acetate was added into eppendorf then sonicated (280 W) for 30 seconds. The elutent was dried under nitrogen stream and dissolved by 25 μL MSTFA. The extracts were derivatized under 200 W microwave condition for 30 seconds and then dried under nitrogen stream. After redissolved by 25 μL EA, the extracts were analysed by GC-MS/MS system. The linearity of the proposed method ranged from 0.025 to 50 ng/mL for 2OH- BP, BP-2 and BP-6; 0.0125 to 18.75 ng/mL for BP-7; 0.05 to 15 ng/mL for HBP; 0.375 to 50 ng/mL for 4-MBC; 0.025 to 25 ng/mL for BP-3; 0.0125 to 50 ng/mL for BP-1; 0.05 to 100 ng/mL for BP-8 and BP-9; 0.0125 to 25 ng/mL for OD-BAPA, OMC and BP-12 with coefficients of determination (R2) above 0.9913. The limits of detection (LODs) and limits of quantification (LOQs) were between 0.26 to 5.3 ng/L and 0.87 to 17.7 ng/L, respectively. The synthesized PPy/MWCNT material can extract analytes with high extraction efficiency and high selectivity. The proposed method can be applied to the determination of trace UV filters in water and urine samples.
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