| Summary: | 碩士 === 國立中興大學 === 生命科學院碩士在職專班 === 102 === The fruit of Sapindus saponaria contains high concentration of saponin with antibacterial and anticancer effects. The aim of this study is to apply the various separations of methanol extract of S.saponaria on different kinds of cancer cells to examine their anticancer activity with MTT assay. The result showed that non-small cell lung carcinoma A549 cell is the most susceptible one for the cytotoxicity of the methanol extract of S.saponaria (ESS1), same as that of the previous study. The ESS1 was then fractioned into ethyl acetate layer (ESS2) and water layer (ESS3). The water fraction, ESS3, of ESS1 was hydrolyzed with KOH followed by the re-extraction of ethyl acetate to obtain ESS4. MTT assay was used again to determine the cytotoxicity on A549 cells with different kinds of extraction layers of S. saponaria. The data appeared that ESS2 showed the best result for cytotoxicity test. Through the flow cytometry analysis, ESS2 can arrest A549 cell growth at G1 phase of the cell cycle. The data of double staining experiment showed that ESS2 induced A549 cell to cell death with the necrosis pathway, same as that of ESS1. ESS1was combined formulation with the essential oil extract of Pelargonium capitatum, induced cell necrosis, or with the essential oil extract of Origanum vulgare, induced cell apoptosis, to A549 cells for synergic effect of treatments. After the MTT assay performance and through the Chou-Talalay equation to obtain CI values, both of the combined formulations observe no synergy effects.
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