Expression of reduced folate carrier in normal canine tissues and lymphomas

• Main Authors: Chun-Yuan Huang, 黃俊元
• Other Authors: Shih-Chieh Chang
• Format: Others
• Language: en_US
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/73724633070960554069

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 102 === Reduced folate carrier (RFC) is the main carrier which transports folates and antifolates into the cell. RFC gene expression in human and rodent tissues had been established, however, it has not been revealed in dogs yet. The aims of the present study were: (1) to construct specific PCR primers for amplification of canine RFC gene; (2) to assess RFC gene expressions in normal canine and lymphomas tissues. Thirty-five kinds of normal canine tissues were collected from five euthanized dogs and twenty-two lymphomas were enrolled. Total RNA were extracted from normal and lymphoma tissues, and were transcripted reversely to complementary DNA, and then were evaluated for RFC expressions using polymerase chain reaction. RFC expression levels were obtained from quantified bands of RFC divided by glyceraldehyde 3-phosphate dehydrogenase. It was shown that RFC transcripts were expressed ubiquitously but various levels. Higher expressions were observed in central nervous and immune system than the median of all tested tissues. Expressions of RFC in normal lymphoid tissues were significantly higher when compared to B- or T-cell lymphomas (P < 0.001). And there were no significant difference among multicentric B-cell lymphomas, multicentric T-cell lymphomas, and cutaneous T-cell lymphomas. We concluded that the ubiquitous distribution with various levels of RFC in 35 kinds of normal canine tissues and malignant tissues. Higher expressions were observed in central nervous, immune, and urinary systems than others. Stomach had the highest RFC expression, followed by cerebellum and brain stem, with very low levels in duodenum and cardiac muscle. And expressions of RFC were significantly lower in all cases of lymphomas when compared to normal lymphatic organs (thymus, palatine tonsil, lymph node), which composed mainly of lymphocytes.