Comparing the expression of different stem cell markersbetween interdental papilla and marginal gingiva

• Main Authors: Fang-ChiaHsu, 許芳嘉
• Other Authors: Yuan Kuo
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/32193962201485893332

碩士 === 國立成功大學 === 口腔醫學研究所 === 102 === In previous papers, we can see that there have been comparative studies between interdental papilla and marginal gingival to look at some specific markers. In this study, we have three aims. First, we utilized human stem cell PCR array to explore the gene expression levels between interdental papilla and marginal gingival and also confirmed this phenomenon by using real-time PCR with a different primer. Next, we confirmed the protein levels by western blotting. Finally, We confirmed clinical expression by IHC and utilized HistoQuest to quantify the immunohistochemistry expression. We found that there was a significant difference in the expression levels of SOX1, ALPI, ALDH1A1, ISL1 and MME between interdental papilla and marginal gingiva. Although the IHC expression of SOX1 did not correlate our PCR array data, we still found that ALPI, ALDH1A1, MME and ISL1 were expressed higher in interdental papilla than in the marginal gingival through real-time PCR, western blot and immunohistochemistry. Therefore, our results show that interdental papilla has higher expression levels of stem cell markers than marginal gingiva. Thus, papilla may be more easily stimulated for growth and may be utilized to treat papilla deficiencies.