| Summary: | 碩士 === 國立成功大學 === 生命科學系 === 102 === Drosophila mesoderm development proceeds in a step-wise fashion. The whole mesoderm is first specified by the expression of a master gene and then subdivided into primordia of different tissues. D-six4 and Eya, a Six family transcription factor and its co-activator, respectively, are expressed in the whole mesoderm and then restricted to the ventral mesoderm. These two genes work together to affect the formation of the ventral mesodermal derivatives, such as fat body and somatic gonad. RNA interference (RNAi) has been shown to be a powerful method to study the function of genes in vivo by silencing endogenous mRNA with double-stranded (ds) RNA. The specification of dorsal mesodermal cell fate has been studied thoroughly while very little is known about the cell fate determination of the ventral mesoderm. In order to identify novel genes involved in Drosophila mesoderm development, I established pPTGal4-six4 transgenic fly lines, which drive specific gene expression in the mesoderm. I screened 46 genes through tissue-specific RNAi experiments and found that CG10591, CG8981, CG3758 and CG11068 may be involved in Drosophila mesoderm development.
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