Establishment of wound array model in Lanyu pigs

• Main Authors: Yu-BinWang, 王鈺斌
• Other Authors: Lynn L.H. Huang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/7jsmy3

碩士 === 國立成功大學 === 生物科技研究所 === 102 === Wound repair is a complex biologic process which is influenced by many factors and compounds. Both in vitro and in vivo models are widely-used in wound healing research. The ideal animal model should reflect human wound healing problems, so it is very important for us to establish the stable and consistent animal models for wound healing studies. The purpose of this study is to establish the wound array model in Lanyu pigs, and use the wound array model as screening platform for drug therapy. In wound creating, the scalpel and dermatome are used to create full-thickness wounds and partial-thickness wounds. The result showed that wound size standard deviation of each creating method is 100%±7.5% and 100%±16.1%. And there is no significant site effect in pig dorsal skin by histological feature, wound closure and wound contraction parameters (p〉0.05). Then, we use these wound healing parameters to analyze the difference between partial- and full- thickness wounds. There were significant differences in wound closure in day 7 and day 9 (p〈0.005) and wound contraction in day 13 (p〈0.001). In addition, we also use Laser Doppler Imager to analyze the blood flow signal during wound healing. Therefore, we suggest that the wound array model in Lanyu pigs which can combine many parameters to estimate for healing quality is a good platform for screening drug therapy.