| Summary: | 碩士 === 國立成功大學 === 分子醫學研究所 === 102 === Escherichia coli. E. coli K1 RS218, an extraintestinal pathogenic E. coli (ExPEC) strain isolated from a neonate with meningitis patient, containing a plasmid named pRS218. Based on the genomic information and protein blast results, we identified 3 potential iron acquisition systems located on the pRS218. Among the 3 operons of pRS218, the fetMP operon encodes a ferrous iron transport system. The eco42-43 operon encodes a potential heme transport system, which may utilize heme-iron as an iron source. The eco60-63 operon has been predicted to be involved in iron acquisition. We constructed an iron-uptake defected MG1655 E. coli K12 strain and utilize the strain to investigate the iron-uptake function of these operons carried by pRS218. The growth results suggest that the fetMP operon may play a more significant role than the eco42-43 and eco60-63 operons in terms of iron uptake. Overall, our results suggest that the pRS218 plasmid may contribute to the iron-uptake ability of RS218, to facilitate bacterial growth in iron-restricted condition when in host as well as to increase the virulence of the bacterial pathogen.
key word: Escherichia coli, iron-uptake gene
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