Effect of different porosity of three-dimensional porous titanium dioxide for adipose stem cells to differentiate into osteoblasts and adipocytes

• Main Authors: Huang, Chun-Hao, 黃鈞皓
• Other Authors: Lin, Chien-Cheng
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/51262109180466091527

碩士 === 國立交通大學 === 材料科學與工程學系所 === 102 === The samples were prepared with various content of sodium chloride and titanium dioxide powder at different volume percent, then sinter the samples at 1300°C for 4 hours for a three-dimensional porous titanium dioxide foam. The adipose stem cells grown on the titanium dioxide foam, then differentiate into osteoblasts cells and adipocytes cells. The study researched the different porosity titanium dioxide foam for the effect of the differentiation of adipose stem cells, used the X-ray diffraction(X-ray diffraction, XRD), scanning electron microscope (SEM) to analysis the crystal structure and surface morphology of the titanium dioxide foam. The XRD results show the sample did not produce other compounds. The SEM result show the holes and the porosity of titanium dioxide foam increase with the volume of sodium chloride. In the part of the culture of adipose stem cell, Fluorescein Diacetate (FDA) fluorescence image could observed the adipose stem cells attached on the titanium dioxide foam, and the multifunctional microplate analyzer calculated the cell count had growth trend, which means that titanium dioxide for adipose stem cells is non-toxic. In the part of the differentiation of adipose stem cell, for the adipose stem cells differentiate into bone cells, quantitative real-time polymerase chain reaction (qPCR) measured the gene expression found in the titanium dioxide foam had poorly differentiated effects compared with the well plate. For the effect of adipose stem cells differentiate into adipose cells on titanium dioxide foam, the adipose cells gene expression on titanium dioxide foam are significantly better than the adipose cells gene expression on well plate. Also the oil droplets staining experiment also confirmed the adipose cells were generated on the titanium dioxide foam and on the well plate. This experiment could be learned that the effect of adipose stem cells differentiate into bone cells on titanium dioxide foam is poor, however the effect of adipose stem cells differentiate into adipose cells on titanium dioxide foam is better than on the well plate, this meant that the titanium dioxide foam suit adipose stem cells to differentiate into adipose cells.