Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system
碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 102 === Biofuel synthesis is of increasing interest because of global energy and environmental problems. Isobutanol is a renewable and clean biofuel. Compared with ethanol, isobutanol has high energy density to burn, low corrosiveness to be stored and long carbon c...
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ndltd-TW-102NCTU55380042016-07-02T04:20:29Z http://ndltd.ncl.edu.tw/handle/10085443455983632432 Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system 利用大腸桿菌在溫度調控系統下提升生質異丁醇發酵產量之研究 Wu, Shao-I 吳劭易 碩士 國立交通大學 分子醫學與生物工程研究所 102 Biofuel synthesis is of increasing interest because of global energy and environmental problems. Isobutanol is a renewable and clean biofuel. Compared with ethanol, isobutanol has high energy density to burn, low corrosiveness to be stored and long carbon chain, which is similar to gasoline. Furthermore, isobutanol can completely replace gasoline without engine modification. Since Escherichia coli can broadly utilize 5-C sugar (xylose, arabinose, mannose) and 6-C sugar (glucose, galactose); therefore, it is a promising host to produce isobutanol. In this study, four genes (alsS, ilvC, ilvD, kivd) were cloned and transformed into E. coli DH5α to direct glycolysis pathway for isobutanol production. In order to improve the yield of isobutanol, a thermo- sensitive ribosome biding site and tetR repressor were used to repress kivd gene expression and overcome the barrier of isobutanol tolerance of E. coli. The result revealed that E. coli harboring four genes overexpression could produce 0.4 g/L (v/v) isobutanol after 48 h cultivation. Moreover, E. coli harboring the modified plasmid with temperature control could achieve 20 g/L isobutanol yield. This result indicates that thermo-sensitive RBS for expressing kivd gene in E. coli may enhance isobutanol production by temperature. Tseng, Ching-Ping 曾慶平 2013 學位論文 ; thesis 85 zh-TW |
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碩士 === 國立交通大學 === 分子醫學與生物工程研究所 === 102 === Biofuel synthesis is of increasing interest because of global energy and environmental problems. Isobutanol is a renewable and clean biofuel. Compared with ethanol, isobutanol has high energy density to burn, low corrosiveness to be stored and long carbon chain, which is similar to gasoline. Furthermore, isobutanol can completely replace gasoline without engine modification. Since Escherichia coli can broadly utilize 5-C sugar (xylose, arabinose, mannose) and 6-C sugar (glucose, galactose); therefore, it is a promising host to produce isobutanol. In this study, four genes (alsS, ilvC, ilvD, kivd) were cloned and transformed into E. coli DH5α to direct glycolysis pathway for isobutanol production. In order to improve the yield of isobutanol, a thermo- sensitive ribosome biding site and tetR repressor were used to repress kivd gene expression and overcome the barrier of isobutanol tolerance of E. coli. The result revealed that E. coli harboring four genes overexpression could produce 0.4 g/L (v/v) isobutanol after 48 h cultivation. Moreover, E. coli harboring the modified plasmid with temperature control could achieve 20 g/L isobutanol yield. This result indicates that thermo-sensitive RBS for expressing kivd gene in E. coli may enhance isobutanol production by temperature.
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author2 |
Tseng, Ching-Ping |
author_facet |
Tseng, Ching-Ping Wu, Shao-I 吳劭易 |
author |
Wu, Shao-I 吳劭易 |
spellingShingle |
Wu, Shao-I 吳劭易 Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system |
author_sort |
Wu, Shao-I |
title |
Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system |
title_short |
Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system |
title_full |
Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system |
title_fullStr |
Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system |
title_full_unstemmed |
Metabolic engineering of Escherichia coli to enhance bio-isobutanol production by temperature regulation system |
title_sort |
metabolic engineering of escherichia coli to enhance bio-isobutanol production by temperature regulation system |
publishDate |
2013 |
url |
http://ndltd.ncl.edu.tw/handle/10085443455983632432 |
work_keys_str_mv |
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