| Summary: | 碩士 === 國立中央大學 === 系統生物與生物資訊研究所 === 102 === Breast cancer incidence and mortality rates haves increased over the years, but due to limitation of current medicines, there were no effective way extending the lives of patients. Although many Chinese ancient books had recorded the benefits of Chinese medicine in terms of cancer treatments by alleviating fever, de-toxication and, eliminating the blood flow by promoting blood circulation, the mainstream medicines still haves not yet approve scientifically their efficacies and use. In this study, we used high-throughput drug screening cytology system (High-throughput Drug Screening System Cytology, HT-DSSC) to screen 60 herbal extracts, and BP004 (The dandelion extract) was the candidate for our study. BP004 was found to inhibit the growth of luminal-A breast cancer cell line MCF-7 by inducing cell apoptosis; however, none of the triple-negative breast cancer cell lines such as MDA-MB 231, HER2-positive breast cancer cell lines MDA-MB-453 and normal mammary epithelial cell H184B5F5/M10 were effected. The extrinsic and intrinsic apoptosis pathways were examined by Western blot. We found that BAX, CASP-7, and FAS proteins were increased in expression while, BCL-2 was decreased expression, indicating cells undergo apoptosis through the intrinsic pathway. Finally, using microarray analysis, genes involved with "cell cycle" and "cellular response to stimulus were also identified". These results indicated that BP004 has is the potential adjuvant drug in the treatment of breast cancer cells. In the future, we will conduct animal experiments, to further confirm the effectiveness of BP004 in the treatment of breast cancer. We use of the technology for high-throughput microarray systems biology to integrate the complex relationships between elements of gene regulation and protein interactions for drug BP004 establish a molecular markers for breast cancer classification.
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