| Summary: | 碩士 === 國立中央大學 === 環境工程研究所 === 102 === Currently, three methodologies, including animal experiments, ex vivo studies in vitro systems of exposure, are generally used to study adverse cellular effects induced by inhalable nanoparticles. Although, comparing to animal experiments, in vitro models lack the ability to account for all intercellular interactions in the cells, in vitro experimental setups are still widely employed due to its relatively low labor and capital cost. In addition, the in vitro studies can avoid the ethical issues provoked by animal experiments. Among the reported in vivo inhalation-exposure systems, the direct air-liquid interface (ALI) exposure method is considered as a more realistic experimental scheme, since its exposure scenario is more close to the real case. However, most of the ALI exposure systems reported in the literatures are not suitable for performing nanoparticle exposure experiments, due to the low collection efficiency of nanoparticles. In addition, very few studies addressed the issue of uniformity of particle deposition pattern over the exposed interface. In this study, a new ESP-type ALI exposure chamber was designed to improve the nanoparticle collection efficiency on the air-liquid exposure interface. The nanoparticle penetration tests were performed to validate the new design of ESP-type ALI chamber. Furthermore, the uniformity of the nanoparticle deposition pattern on the exposure interface is investigated by means of inspecting deposition pattern of fluorescein salt nanoparticle in the inner surfaces of the ALI chamber. At last, a semi-theoretical/semi-empirical expression was proposed to accurately estimate the exposed dose of nanoparticles.
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