Mirror-assisted Tomographic Phase Microscopy
碩士 === 國立中央大學 === 光電科學與工程學系 === 102 === The main purpose of this study is to improve tomographic phase microscopy (TPM). The method is to increase mirror and off-axis holographic structure in the novel system. Not only saving functions of the traditional TPM, but also reducing system complexity and...
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2014
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ndltd-TW-102NCU056140172019-05-15T21:13:46Z http://ndltd.ncl.edu.tw/handle/32t984 Mirror-assisted Tomographic Phase Microscopy 鏡像輔助斷層掃描相位顯微鏡 Wei-Mao Hsu 徐維懋 碩士 國立中央大學 光電科學與工程學系 102 The main purpose of this study is to improve tomographic phase microscopy (TPM). The method is to increase mirror and off-axis holographic structure in the novel system. Not only saving functions of the traditional TPM, but also reducing system complexity and scanning time. The first, the system uses the concept of off-axis digital holography to obtain phase projections of sample in different directions, and the wrapped phase will be unwrapped to successive phase distribution by phase unwrapping algorithm. Finally, the three-dimensional refractive index distribution of the reconstructed object by filtered backprojection (FBP)algorithm. There are simulation and experiment in the study. In the simulation, the various algorithms about image reconstruction will be established in the reconstruction process for processing the raw data captured by the experiment. In the experiment, the first is the construction of mirror-assisted TPM system. Then using beads of the known diameter and refractive index as a sample to obtain three-dimensional refractive index distribution of the beads for verifying the novel system and algorithms. The system will also be applied to measure the three-dimensional refractive index distribution of cell. Szu-Yu Chen Ching-Cherng Sun 陳思妤 孫慶成 2014 學位論文 ; thesis 83 zh-TW |
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碩士 === 國立中央大學 === 光電科學與工程學系 === 102 === The main purpose of this study is to improve tomographic phase microscopy (TPM). The method is to increase mirror and off-axis holographic structure in the novel system. Not only saving functions of the traditional TPM, but also reducing system complexity and scanning time. The first, the system uses the concept of off-axis digital holography to obtain phase projections of sample in different directions, and the wrapped phase will be unwrapped to successive phase distribution by phase unwrapping algorithm. Finally, the three-dimensional refractive index distribution of the reconstructed object by filtered backprojection (FBP)algorithm.
There are simulation and experiment in the study. In the simulation, the various algorithms about image reconstruction will be established in the reconstruction process for processing the raw data captured by the experiment. In the experiment, the first is the construction of mirror-assisted TPM system. Then using beads of the known diameter and refractive index as a sample to obtain three-dimensional refractive index distribution of the beads for verifying the novel system and algorithms. The system will also be applied to measure the three-dimensional refractive index distribution of cell.
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author2 |
Szu-Yu Chen |
author_facet |
Szu-Yu Chen Wei-Mao Hsu 徐維懋 |
author |
Wei-Mao Hsu 徐維懋 |
spellingShingle |
Wei-Mao Hsu 徐維懋 Mirror-assisted Tomographic Phase Microscopy |
author_sort |
Wei-Mao Hsu |
title |
Mirror-assisted Tomographic Phase Microscopy |
title_short |
Mirror-assisted Tomographic Phase Microscopy |
title_full |
Mirror-assisted Tomographic Phase Microscopy |
title_fullStr |
Mirror-assisted Tomographic Phase Microscopy |
title_full_unstemmed |
Mirror-assisted Tomographic Phase Microscopy |
title_sort |
mirror-assisted tomographic phase microscopy |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/32t984 |
work_keys_str_mv |
AT weimaohsu mirrorassistedtomographicphasemicroscopy AT xúwéimào mirrorassistedtomographicphasemicroscopy AT weimaohsu jìngxiàngfǔzhùduàncéngsǎomiáoxiāngwèixiǎnwēijìng AT xúwéimào jìngxiàngfǔzhùduàncéngsǎomiáoxiāngwèixiǎnwēijìng |
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1719111595956633600 |