Mirror-assisted Tomographic Phase Microscopy

• Main Authors: Wei-Mao Hsu, 徐維懋
• Other Authors: Szu-Yu Chen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/32t984

碩士 === 國立中央大學 === 光電科學與工程學系 === 102 === The main purpose of this study is to improve tomographic phase microscopy (TPM). The method is to increase mirror and off-axis holographic structure in the novel system. Not only saving functions of the traditional TPM, but also reducing system complexity and scanning time. The first, the system uses the concept of off-axis digital holography to obtain phase projections of sample in different directions, and the wrapped phase will be unwrapped to successive phase distribution by phase unwrapping algorithm. Finally, the three-dimensional refractive index distribution of the reconstructed object by filtered backprojection (FBP)algorithm. There are simulation and experiment in the study. In the simulation, the various algorithms about image reconstruction will be established in the reconstruction process for processing the raw data captured by the experiment. In the experiment, the first is the construction of mirror-assisted TPM system. Then using beads of the known diameter and refractive index as a sample to obtain three-dimensional refractive index distribution of the beads for verifying the novel system and algorithms. The system will also be applied to measure the three-dimensional refractive index distribution of cell.