| Summary: | 碩士 === 國立嘉義大學 === 獸醫學系研究所 === 102 === Abstract
Salmonella infection is an important disease in pigs and a zoonotic disease in human. Infection can be caused by pig livers due to the habit of eating organs in Taiwan. The antimicrobial resistance in Salmonella spp. becomes an issue during these years. This study is aimed to discuss the relationship of Salmonella infection between discarding milk spot livers and normal livers from slaughter pigs. This study was conducted from October 2011 to September 2012, collecting 651 discarding milk spot livers and 80 normal livers from slaughterhouse in the Yun-Chia-Nan areas. Bacterial isolation, histopathological examination and antimicrobial resistance percentage Salmonella spp. were taken to identify the prevalence of Salmonella spp. Forty-seven (7.22 %) and 7 (8.75 %) strains of Salmonella spp. were isolated from 651 milk spot livers 80 normal livers, respectively. The 54 strains of Salmonella included 55.56 % (30/54) S. Typhymurium, 11.11 % (6/54) S. Livingstone var. 14 +, 11.11 % (6/54) S. Derby, 5.56 % (3/54) S. Anatum, 3.70 % (2/54) S. London, 3.70 % (2/54) S. Newport isolation, 1.85% (1/54) S. Brunei, 1.85% (1/54) S. Choleraesuis, 1.85% (1/54) S. Hadar and 2 strains of unknown Salmonella serotype. Paratyphoid nodules were found in 15 (2.30 %) liver samples from discarding milk spot livers and 2 (2.50 %) in normal liver samples. The histopathological findings of 651 milk spot livers included 100 % (651/651) eosinophile infiltration, 96.01 % (625/651) biliary hyperplasia, 95.55 % (622/651) bridge fibrosis, 85.10 % (554/651) connective tissue hyperplasia, 48.69 % (317/651) focal necrosis and 24.58 % (160/651) hemorrhage. In 54 strains of Salmonella spp. isolated in this study, 92.59 % were resistance to amoxicillin, 92.59 % were to lincospectin, 88.89 % were to ampicillin, 85.19 % were to oxytetracycline, 81.48 % were to florfenicol, 74.07 %were to doxycycline, 62.96 % were to colistin, 61.11 % were to sulphamethoxazole/trimethoprim, 33.33 % were to gentamicin and 24.07 % were to flumequine. Isolation of Salmonella and paratyphoid nodules were both showed in discarding milk spot livers and normal livers. Highly and multi-drug resistant to several antimicrobial agents in this study should be attended. In present, the most popular Salmonella serotype in Taiwan is Salmonella Typhimurium. The farm manager should improve the biological safety and managements of the farm to reduce the stress and to control the pigs from Salmonella. Also notice to prevent the salmonellosis in public health.
Keywords: Salmonella,antimicrobial resistance, prevalence, slaughter pig, discarding milk spot liver
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