Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas

碩士 === 國立嘉義大學 === 獸醫學系研究所 === 102 === Abstract Salmonella infection is an important disease in pigs and a zoonotic disease in human. Infection can be caused by pig livers due to the habit of eating organs in Taiwan. The antimicrobial resistance in Salmonella spp. becomes an issue during these years....

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Main Authors: Pu-Yi Jane, 簡朴毅
Other Authors: Dan-Yuan Lo, Ph. D.
Format: Others
Language:zh-TW
Online Access:http://ndltd.ncl.edu.tw/handle/02972973941397108622
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spelling ndltd-TW-102NCYU55410122016-03-09T04:30:46Z http://ndltd.ncl.edu.tw/handle/02972973941397108622 Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas 雲嘉南地區屠宰豬廢棄乳斑肝沙門氏桿菌分離、組織病理變化、盛行率及抗藥性之探討 Pu-Yi Jane 簡朴毅 碩士 國立嘉義大學 獸醫學系研究所 102 Abstract Salmonella infection is an important disease in pigs and a zoonotic disease in human. Infection can be caused by pig livers due to the habit of eating organs in Taiwan. The antimicrobial resistance in Salmonella spp. becomes an issue during these years. This study is aimed to discuss the relationship of Salmonella infection between discarding milk spot livers and normal livers from slaughter pigs. This study was conducted from October 2011 to September 2012, collecting 651 discarding milk spot livers and 80 normal livers from slaughterhouse in the Yun-Chia-Nan areas. Bacterial isolation, histopathological examination and antimicrobial resistance percentage Salmonella spp. were taken to identify the prevalence of Salmonella spp. Forty-seven (7.22 %) and 7 (8.75 %) strains of Salmonella spp. were isolated from 651 milk spot livers 80 normal livers, respectively. The 54 strains of Salmonella included 55.56 % (30/54) S. Typhymurium, 11.11 % (6/54) S. Livingstone var. 14 +, 11.11 % (6/54) S. Derby, 5.56 % (3/54) S. Anatum, 3.70 % (2/54) S. London, 3.70 % (2/54) S. Newport isolation, 1.85% (1/54) S. Brunei, 1.85% (1/54) S. Choleraesuis, 1.85% (1/54) S. Hadar and 2 strains of unknown Salmonella serotype. Paratyphoid nodules were found in 15 (2.30 %) liver samples from discarding milk spot livers and 2 (2.50 %) in normal liver samples. The histopathological findings of 651 milk spot livers included 100 % (651/651) eosinophile infiltration, 96.01 % (625/651) biliary hyperplasia, 95.55 % (622/651) bridge fibrosis, 85.10 % (554/651) connective tissue hyperplasia, 48.69 % (317/651) focal necrosis and 24.58 % (160/651) hemorrhage. In 54 strains of Salmonella spp. isolated in this study, 92.59 % were resistance to amoxicillin, 92.59 % were to lincospectin, 88.89 % were to ampicillin, 85.19 % were to oxytetracycline, 81.48 % were to florfenicol, 74.07 %were to doxycycline, 62.96 % were to colistin, 61.11 % were to sulphamethoxazole/trimethoprim, 33.33 % were to gentamicin and 24.07 % were to flumequine. Isolation of Salmonella and paratyphoid nodules were both showed in discarding milk spot livers and normal livers. Highly and multi-drug resistant to several antimicrobial agents in this study should be attended. In present, the most popular Salmonella serotype in Taiwan is Salmonella Typhimurium. The farm manager should improve the biological safety and managements of the farm to reduce the stress and to control the pigs from Salmonella. Also notice to prevent the salmonellosis in public health. Keywords: Salmonella,antimicrobial resistance, prevalence, slaughter pig, discarding milk spot liver Dan-Yuan Lo, Ph. D. Hung-Chih Kuo, Ph. D. Chiou-Lin Chen, Ph. D. 羅登源 博士 郭鴻志 博士 陳秋麟 博士 學位論文 ; thesis 68 zh-TW
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language zh-TW
format Others
sources NDLTD
description 碩士 === 國立嘉義大學 === 獸醫學系研究所 === 102 === Abstract Salmonella infection is an important disease in pigs and a zoonotic disease in human. Infection can be caused by pig livers due to the habit of eating organs in Taiwan. The antimicrobial resistance in Salmonella spp. becomes an issue during these years. This study is aimed to discuss the relationship of Salmonella infection between discarding milk spot livers and normal livers from slaughter pigs. This study was conducted from October 2011 to September 2012, collecting 651 discarding milk spot livers and 80 normal livers from slaughterhouse in the Yun-Chia-Nan areas. Bacterial isolation, histopathological examination and antimicrobial resistance percentage Salmonella spp. were taken to identify the prevalence of Salmonella spp. Forty-seven (7.22 %) and 7 (8.75 %) strains of Salmonella spp. were isolated from 651 milk spot livers 80 normal livers, respectively. The 54 strains of Salmonella included 55.56 % (30/54) S. Typhymurium, 11.11 % (6/54) S. Livingstone var. 14 +, 11.11 % (6/54) S. Derby, 5.56 % (3/54) S. Anatum, 3.70 % (2/54) S. London, 3.70 % (2/54) S. Newport isolation, 1.85% (1/54) S. Brunei, 1.85% (1/54) S. Choleraesuis, 1.85% (1/54) S. Hadar and 2 strains of unknown Salmonella serotype. Paratyphoid nodules were found in 15 (2.30 %) liver samples from discarding milk spot livers and 2 (2.50 %) in normal liver samples. The histopathological findings of 651 milk spot livers included 100 % (651/651) eosinophile infiltration, 96.01 % (625/651) biliary hyperplasia, 95.55 % (622/651) bridge fibrosis, 85.10 % (554/651) connective tissue hyperplasia, 48.69 % (317/651) focal necrosis and 24.58 % (160/651) hemorrhage. In 54 strains of Salmonella spp. isolated in this study, 92.59 % were resistance to amoxicillin, 92.59 % were to lincospectin, 88.89 % were to ampicillin, 85.19 % were to oxytetracycline, 81.48 % were to florfenicol, 74.07 %were to doxycycline, 62.96 % were to colistin, 61.11 % were to sulphamethoxazole/trimethoprim, 33.33 % were to gentamicin and 24.07 % were to flumequine. Isolation of Salmonella and paratyphoid nodules were both showed in discarding milk spot livers and normal livers. Highly and multi-drug resistant to several antimicrobial agents in this study should be attended. In present, the most popular Salmonella serotype in Taiwan is Salmonella Typhimurium. The farm manager should improve the biological safety and managements of the farm to reduce the stress and to control the pigs from Salmonella. Also notice to prevent the salmonellosis in public health. Keywords: Salmonella,antimicrobial resistance, prevalence, slaughter pig, discarding milk spot liver
author2 Dan-Yuan Lo, Ph. D.
author_facet Dan-Yuan Lo, Ph. D.
Pu-Yi Jane
簡朴毅
author Pu-Yi Jane
簡朴毅
spellingShingle Pu-Yi Jane
簡朴毅
Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas
author_sort Pu-Yi Jane
title Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas
title_short Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas
title_full Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas
title_fullStr Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas
title_full_unstemmed Studies on Histopathological Changes, Prevalence and Antimicrobial Resistance of Salmonella spp. Isolated from Discarding Milk Spot Livers of Slaughter Pig at Yun-Chia-Nan Areas
title_sort studies on histopathological changes, prevalence and antimicrobial resistance of salmonella spp. isolated from discarding milk spot livers of slaughter pig at yun-chia-nan areas
url http://ndltd.ncl.edu.tw/handle/02972973941397108622
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