Investigating the fate of human adipose-derived stem cells on the treatment of type II diabetes by STZ-NA-induced diabetic mice

• Main Authors: Wan-Ting Huang, 黃琬婷
• Other Authors: Tzyy-Wen Chiou
• Format: Others
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/b8996f

碩士 === 國立東華大學 === 生命科學系 === 102 === Due to major changes in lifestyle and diet an increasing number of the population are becoming diabetic. Among the four types of diabetes, the type II diabetes is the most prevalent with 95% of all diabetes around the world; however, its effective treatment is still an unmet medical need.Adipose-derived stem cells can be easily obtained from donors using fat extraction by minimally invasive surgery. They are highly proliferative, multipotent and able to regulate immune and inflammatory responses.The objective of this study is to investigate the ability of human adipose-derived stem cells (hADSCs) to differentiate into pancreatic β-like cells and to examine the fate of hADSCs transplanted in STZ-NA-induced type II diabetic mice. In vitro experiments showed hADSCs could be induced to pancreatic β-like cells by three different strategies. Among them, the method combined with co-culture and differentiation medium was shown to be the most effective for the induction. In the mice model, CM-Dil dye was used to label the hADSCs for the purpose of tracking the cells. Cell transplantation was performed by tail vein injection of labeled hADSCs. By the microscopic observations on tissues sections from mice, it was found that transplanted hADSCs appeared mainly in pancreas and liver during the first month after transplantation. The labeled cells in the targeted organs were enumerated during the first two months after transplantation. The infiltration of immune cells to pancreatic tissue was decreased and the damage of the islet cells was ameliorated in the hADSCs transplanted mice compared to the sham treatment. Based on these results, it could be concluded that the hADSCs can differentiate into pancreatic β-like cells and have great potential in the application of type II diabetes treatment.