| Summary: | 碩士 === 國防醫學院 === 生物化學研究所 === 102 === Mammary gland development is regulated by hormones and grpwth factors. When mammary gland undergoes development, the tissue structures requires remodeling through the protease network. Matriptese, a type II transmembrane serine protease, can be activated by S1P in serum and it can further remodel extracellular matrix. Our recent results also suggest that matriptase plays an important role in epidermis differentiation. Therefore we hypothesize that matriptase could regulate mammary gland morphogenesis and differentiation during mammary gland development. To confirm the differentiation of mammary gland, we use the different biomarkers of mammary gland differentiation: α6 integrin, MUC1, keratin 8, keratin 14, keratin 18, keratin 19, vimentin and E-cadherin. Our results demonstrate that matriptase could change the expression level of different mammary gland differentiation markers. Since 184 A1N4 cell line includes several morphological cells, we performed serial dilution to pick several subclones and examined certain biomarker level in those subclones. According to our results, we draw some conclusions. First, activated matriptase decrease, cell morphogenesis could de delayed. Next, the expression of the mammary gland differentiation biomarker will be changed with cell morphogenesis and those biomarker expression could be affectd by activated matriptase. Therefore , matriptase may be involved in mammary differentiation.
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