Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak

• Main Authors: Jun-Jie Chen, 陳俊傑
• Other Authors: Chiu-Min Cheng
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/d8w7u2

碩士 === 國立高雄海洋科技大學 === 水產養殖研究所 === 102 === NNV has caused serious economic losses in the global aquaculture. To decrease the NNV concentration in the farming environment, we developed the NNV-binding protein display bacteria in which a NNV-binding protein fuse to c termini of lppompA, a gram negative membrane display system, for efficient display protein on bacterial surface. Moreover, several studies have reported that innate immunity effector Mx proteins can bind with NNV, therefore, in this study the Mx protein was fuse to C termini of lppompA to generate lpp-Mx, then transformed it into bacteria to form lpp-Mx/BL21. We demonstrated that lpp-Mx/BL21 expressed Mx on bacterial surface, and the lpp-Mx/BL21 could bind with NNV and reduce the NNV concentration in the culture medium. These results suggest that lppompA fused with Mx produces the NNV-binding bacteria which can remove NNV from farming environment and prevent NNV spread and improve the survival rate of grouper Juveniles.