Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak
碩士 === 國立高雄海洋科技大學 === 水產養殖研究所 === 102 === NNV has caused serious economic losses in the global aquaculture. To decrease the NNV concentration in the farming environment, we developed the NNV-binding protein display bacteria in which a NNV-binding protein fuse to c termini of lppompA, a gram negative...
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ndltd-TW-102NKIM00860082019-05-15T21:24:14Z http://ndltd.ncl.edu.tw/handle/d8w7u2 Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak 細菌膜表現神經壞死病毒結合蛋白以預防神經壞死症爆發 Jun-Jie Chen 陳俊傑 碩士 國立高雄海洋科技大學 水產養殖研究所 102 NNV has caused serious economic losses in the global aquaculture. To decrease the NNV concentration in the farming environment, we developed the NNV-binding protein display bacteria in which a NNV-binding protein fuse to c termini of lppompA, a gram negative membrane display system, for efficient display protein on bacterial surface. Moreover, several studies have reported that innate immunity effector Mx proteins can bind with NNV, therefore, in this study the Mx protein was fuse to C termini of lppompA to generate lpp-Mx, then transformed it into bacteria to form lpp-Mx/BL21. We demonstrated that lpp-Mx/BL21 expressed Mx on bacterial surface, and the lpp-Mx/BL21 could bind with NNV and reduce the NNV concentration in the culture medium. These results suggest that lppompA fused with Mx produces the NNV-binding bacteria which can remove NNV from farming environment and prevent NNV spread and improve the survival rate of grouper Juveniles. Chiu-Min Cheng 鄭秋敏 2014 學位論文 ; thesis 42 zh-TW |
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碩士 === 國立高雄海洋科技大學 === 水產養殖研究所 === 102 === NNV has caused serious economic losses in the global aquaculture. To decrease the NNV concentration in the farming environment, we developed the NNV-binding protein display bacteria in which a NNV-binding protein fuse to c termini of lppompA, a gram negative membrane display system, for efficient display protein on bacterial surface. Moreover, several studies have reported that innate immunity effector Mx proteins can bind with NNV, therefore, in this study the Mx protein was fuse to C termini of lppompA to generate lpp-Mx, then transformed it into bacteria to form lpp-Mx/BL21. We demonstrated that lpp-Mx/BL21 expressed Mx on bacterial surface, and the lpp-Mx/BL21 could bind with NNV and reduce the NNV concentration in the culture medium. These results suggest that lppompA fused with Mx produces the NNV-binding bacteria which can remove NNV from farming environment and prevent NNV spread and improve the survival rate of grouper Juveniles.
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author2 |
Chiu-Min Cheng |
author_facet |
Chiu-Min Cheng Jun-Jie Chen 陳俊傑 |
author |
Jun-Jie Chen 陳俊傑 |
spellingShingle |
Jun-Jie Chen 陳俊傑 Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak |
author_sort |
Jun-Jie Chen |
title |
Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak |
title_short |
Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak |
title_full |
Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak |
title_fullStr |
Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak |
title_full_unstemmed |
Expression of Nervous necrosis virus (NNV) binding protein on surface of bacteria prevents NNV disease outbreak |
title_sort |
expression of nervous necrosis virus (nnv) binding protein on surface of bacteria prevents nnv disease outbreak |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/d8w7u2 |
work_keys_str_mv |
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