Molecular cloning、gene expression and phylogenetic analysis of LLP20, in lily pollen

• Main Authors: CHENG, YI-JU, 鄭亦茹
• Other Authors: HUANG, JONG-CHIN
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/6qr95g

碩士 === 國立屏東教育大學 === 化學生物系碩士班 === 102 === Lily （Lilium longiflorum） pollen cDNA LLP20 was obtained from the suppression subtractive hybridization cDNA library of lily anther at the desiccation stage. Sequence alignment of LLP20 cDNA shows the high sequence identity compared to the ATP-binding cassette (ABC) transporter superfamily in Arabidopsis thaliana. ABC protein superfamily is a membrane protein family of functional versatility, which is more encoded by the plant genome than genomes of human or fly (Drosophila melanogaster). Previous researches suggest that plant ABC transporter protein participate in some essential plant processes, including polar auxin transport, stomatal regulation, lipid catabolism, and heavy metal tolerance…etc. However, their major physiological function in mature pollen is unclear. To characterize LLP20, we aim to obtain the full-length sequence of LLP20 clone by 5’RACE and examine its expression pattern by RT-PCR. Furthermore, we will perform the localization analysis and transient expression assay to decipher the location and function of LLP20.