| Summary: | 碩士 === 國立屏東科技大學 === 生物科技系所 === 102 === Alpinia officinarum Hance. ( Zingiberaceae ) is a perennial herb that was widely
cultivated in Guangdong, Guangxi, and Taiwan. A. officinarum is also called Liang zang,
Gau liang zang, Fong zang, and Hai lang zang. Previous studies have demonstrated that A.
officinarum rhizomes contain a lot of chemical substances of flavonoids and
diarylheptanoids. with anti-cancer, anti-virus, anti-bacterial, anti-inflammatory,
anti-mutagenic and anti-oxidant activities.
In this study, the air-dried rhizomes of A. officinarum were extracted with methanol.
The MeOH extract was partitioned by ethyl acetate and butanol with water, respectively, to
give EtOAc, n-BuOH and water layers.
The extract of each layer was subjected to screen biological acitrvities and the result
showed that the EtOAc layer of A. officinarum rhizomes have significant anti-oxidant and
cytotoxic activities. Furthermore, these fractions from the EtOAc layer were purified by
repeated column chromatography, high performance liquid chromatography and
recrystallization. To obtain 23 compounds including one steroid, three flavonoids, one
IV
monoterpenoid, two sesquiterpenoids, two aromatics and fourteen diarylheptanoids. Their
structures were elucidated by spectroscopic analysis including UV, EI-MS and NMR
techniques.
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