| Summary: | 碩士 === 國立屏東科技大學 === 生物科技系所 === 102 === Taiwan Red False-Cypress (Chamaecyparis formosensis) is an endemic tree that grows in Taiwan’s central mountains at moderate to high altitudes. This plant is famous for its high-grade wood quality with beautiful texture and attractive fragrance. While Red Lemongrass (Cymbopogon nardus (L) Rendle), a Poaceae, has been broadly used in food, drinks, perfumery, body care products and soap manufacture. In this study, essential oils from both plant were examined for their composition, in vitro and in vivo activities by using GC-MS method, DPPH free radical scavenging activity, acute toxicity assay through oral feeding on BALB/c mice and cytotoxicity against RAW 264.7 (murine macrophage) and FL83B (human hepatocytes) cell lines. There were 26 compounds found in C. formosensis essential oil, distilled from root, trunk and branches, that mainly consist of cadinene (23.53%, cyclopentanol (12.99%) and T-muurolol (11.28%). Meanwhile C. nardus essential oil, distilled from leaf and stem, mainly consist of (+)-trans,trans-5-Caranol (55.07%) and elemol (13.69%). Antioxidant activity of both oil were determined by using DPPH assay, the result showed that 3 different oil concentration 5, 10 and 50 μg/ml of C. nardus (7.73±2.63%; 13.43±4.16% and 24.33±1.66% respectively) showed higher radical cavenging activity than C. formosensis (49.21±3.39%; 61.74±4.21% and 91.49±0.72% respectively). While acute toxicity assay in BALB/c mice showed that C. formosensis and C. nardus showed LD50 of 2290.87 ± 684.28 and 2511.89 ± 791.51 mg/kg body weight respectively. These results suggest that both essential oil have low toxicity for oral feeding on BALB-c mice. Cytotoxicity test against two different cell lines, indicate that C. formosensis essential oil is considered more toxic on RAW 264.7 cells than on FL83B cells while C. nardus essential oil is considered less toxic on RAW 264.7 cells than on FL83B cells.
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