Development of a virus-like particle vaccine against foot and mouth disease

• Main Author: 王偉綸
• Other Authors: Chung, yao-chi
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/02944448722899138760

碩士 === 國立屏東科技大學 === 動物疫苗科技研究所 === 102 === Foot and mouth disease (FMD) is an acute infectious disease of cloven-hoofed animals. The outbreak of the disease severely limits trade in animals and animal products, then indirectly causes economic and industrial losses. Foot and mouth disease virus (FMDV), a single-stranded, positive-sense RNA virus with a 8.5 kb genome, is the pathogen of FMD. FMDV belongs to genus Aphthovirus within the family Picornaviridae. There are seven serotypes of FMDV including O, A, C, Asia 1, South African Territories (SAT) 1, 2 and 3. Currently the major strategy to control outbreaks of FMD is using inactivated vaccine. Because of biosafety issues, there are no related manufacturers in Taiwan. In addition, the mutation in FMDV is quite often and quick, hence the imported vaccines may be invalid. So we must develop appropriate vaccines that suitable for production and vaccination in Taiwan. The virus-like particles (VLP) has the potential to be a new generation of FMD vaccine. The structure of FMDV capsid is icosahedral, and the capsid protein is composed of the processed P1 protein. The P1 protein is processed by viral protease 3C to produce the structural proteins VP0, VP1 and VP3. These proteins then self-assemble to form virus capsid. In this study, the FMDV P1 and 3C genes were cloned and inserted into the baculovirus, then the recombinant baculovirus virus infected insect cells to produces VLP. At present, we had found that VP1 was detected by Western blot, which might confirmed the successfully expression of recombinant P12A and 3C proteins. The antigenicity of VLP was confirmed by animal experiment. After detecting the titers of anti-FMDV antibody and T-cell proliferation, the data shown that immunization of mice with VLP elicited a strong immue response. These recombinant virus-like particles are potentially useful in development of FMDV vaccines.