The distribution of endocrine disrupting chemicals and vitellogenin expression of male Oreochromis niloticus in river

• Main Authors: Hsu-Chin Kuo, 郭旭芩
• Other Authors: Chi-Ying Hsieh
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/98f5b4

碩士 === 國立屏東科技大學 === 環境工程與科學系所 === 102 === The distributions of endocrine disruptors were conducted in the riverine environment in many reports. However, little is known about the induction of vitellogenin in male fish by estrogenic chemicals in Taiwan’s river. This study aimed to determine the concentrations of endocrine disrupting chemicals: estrone(E1), 17β-estradiol (E2), estriol (E3), 17α-ethinylestradiol (EE2), diethylstilbestrol (DES), nonylphenol (NP), octylphenol (OP) and bisphenol-A (BPA) in upstream of wastewater treatment plant (site A), wastewater treatment plant effluent (site B), 0.1 km from the outfall of the wastewater treatment plant (site C) and downstream of the wastewater treatment plant (site D) of the river in Southern Taiwan. In addition, vitellogenin expression of the male Oreochromis niloticus using reverse transcription-polymerase chain reaction (RT-PCR) system have also investigated. The result indicated the elevated VTG response shown in the four sampling sites along the river were 26% (n=31), 0% (n=21), 6% (n=17) and 50% (n=22) from upstream to downstream, respectively. The average concentrations in samples collected, from sites upstream and downstream of WWTP, were 5.04 ± 3.79 and 4.60 ± 1.87 (E1), 41.3±49.3 and 18.7±29.1 (NP), 3.22±1.30 and 7.83 ± 10.3 (OP) and 5.04±3.79 and 12.9 ± 24.2 (BPA) ng/L, respectively. More compounds were detected than the other two sampling sites may related to the industrial and locally farmers’ markets wastewater discharged. Only two of the eight compounds, NP and BPA, were detected in site B and the average concentrations were 7.05 ± 10.3 and 3.88 ± 2.47 ng/L. These compounds were also detected in site C with the concentrations of 26.1± 65.4(NP), and 4.53±3.00 (BPA) and in addition, 4.04±2.90 (OP) ng/L. Compounds 17β-estradiol (E2), 17α-ethinylestradiol (EE2), estriol(E3), diethylstilbestrol (DES) were not detected from samples at various sampling sites during the sampling events. Our results demonstrated a parallel pattern that higher detecting frequency of the endocrine disruptors to the VTG production in male fish collected. The results indicated the possibility of that these chemicals probably account for the estrogenic activity in this river while the fish exposed in the low concentrations of alkylphenolic mixture. Therefore, the vitellogenesis of common species can be a useful biomarker to evaluate the impact of endocrine disrupting chemicals in the environment.