Exercise protein irisin promotes angiogenesis in endothelial cells
碩士 === 國立中山大學 === 生物醫學研究所 === 102 === Myokinesis a critical cytokines in organism major secretes from muscle cells after doing exercise. Myokines involved in angiogenesis, tissue regenerate and repair, preserved normal functions of body, affected immune modulation and develop of embryos. Boströmet a...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | en_US |
Published: |
2014
|
Online Access: | http://ndltd.ncl.edu.tw/handle/63e9pg |
id |
ndltd-TW-102NSYS5114117 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-102NSYS51141172019-05-15T21:32:51Z http://ndltd.ncl.edu.tw/handle/63e9pg Exercise protein irisin promotes angiogenesis in endothelial cells 運動蛋白FNDC5/irisin促進內皮細胞血管新生之功能 Hsuan-Yi Hsiao 蕭亘邑 碩士 國立中山大學 生物醫學研究所 102 Myokinesis a critical cytokines in organism major secretes from muscle cells after doing exercise. Myokines involved in angiogenesis, tissue regenerate and repair, preserved normal functions of body, affected immune modulation and develop of embryos. Boströmet al. found a new myokine and called FNDC5 in 2012. FNDC5 was founded possessing a ability that can induce White Adipose Tissue becoming Brown Adipose Tissue to lose weight and slow down the symptom of diabetes through increase burning calories. In the resent reports, there is no direct describe that whether FNDC5 can regulates angiogenesis and wound healing. Recombinant FNDC5 (Fibronectin type III domain-containing protein 5) was expressed and purified from E. coli with a molecular weight of 25 kDa. By using cultured endothelial cells, it was found that recombinant FNDC5 stimulated the proliferation, migration and tube formation of endothelial cells. Moreover, FNDC5 promotes vessels sprouting in aortic rings assay and in transgenic Tg(fli-1:EGFP)y1zebrafish. Finally, FNDC5 application enhanced wound healing in diabetic rats. Mechanism studies showed FNDC5 induced the expression of vascular the endothelial growth factor (VEGF)/vascular endothelial growth factor receptor 2 (VEGFR2/FLK-1) pathway and activated the downstream Erk and p38 signaling in endothelial cells. Therefore, this study unveils the novel pro-angiogenic the function and mechanism of FNDC5 in endothelial cells. Ming-Hong Tai 戴明泓 2014 學位論文 ; thesis 63 en_US |
collection |
NDLTD |
language |
en_US |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 國立中山大學 === 生物醫學研究所 === 102 === Myokinesis a critical cytokines in organism major secretes from muscle cells after doing exercise. Myokines involved in angiogenesis, tissue regenerate and repair, preserved normal functions of body, affected immune modulation and develop of embryos. Boströmet al. found a new myokine and called FNDC5 in 2012. FNDC5 was founded possessing a ability that can induce White Adipose Tissue becoming Brown Adipose Tissue to lose weight and slow down the symptom of diabetes through increase burning calories. In the resent reports, there is no direct describe that whether FNDC5 can regulates angiogenesis and wound healing. Recombinant FNDC5 (Fibronectin type III domain-containing protein 5) was expressed and purified from E. coli with a molecular weight of 25 kDa. By using cultured endothelial cells, it was found that recombinant FNDC5 stimulated the proliferation, migration and tube formation of endothelial cells. Moreover, FNDC5 promotes vessels sprouting in aortic rings assay and in transgenic Tg(fli-1:EGFP)y1zebrafish. Finally, FNDC5 application enhanced wound healing in diabetic rats. Mechanism studies showed FNDC5 induced the expression of vascular the endothelial growth factor (VEGF)/vascular endothelial growth factor receptor 2 (VEGFR2/FLK-1) pathway and activated the downstream Erk and p38 signaling in endothelial cells. Therefore, this study unveils the novel pro-angiogenic the function and mechanism of FNDC5 in endothelial cells.
|
author2 |
Ming-Hong Tai |
author_facet |
Ming-Hong Tai Hsuan-Yi Hsiao 蕭亘邑 |
author |
Hsuan-Yi Hsiao 蕭亘邑 |
spellingShingle |
Hsuan-Yi Hsiao 蕭亘邑 Exercise protein irisin promotes angiogenesis in endothelial cells |
author_sort |
Hsuan-Yi Hsiao |
title |
Exercise protein irisin promotes angiogenesis in endothelial cells |
title_short |
Exercise protein irisin promotes angiogenesis in endothelial cells |
title_full |
Exercise protein irisin promotes angiogenesis in endothelial cells |
title_fullStr |
Exercise protein irisin promotes angiogenesis in endothelial cells |
title_full_unstemmed |
Exercise protein irisin promotes angiogenesis in endothelial cells |
title_sort |
exercise protein irisin promotes angiogenesis in endothelial cells |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/63e9pg |
work_keys_str_mv |
AT hsuanyihsiao exerciseproteinirisinpromotesangiogenesisinendothelialcells AT xiāogènyì exerciseproteinirisinpromotesangiogenesisinendothelialcells AT hsuanyihsiao yùndòngdànbáifndc5irisincùjìnnèipíxìbāoxuèguǎnxīnshēngzhīgōngnéng AT xiāogènyì yùndòngdànbáifndc5irisincùjìnnèipíxìbāoxuèguǎnxīnshēngzhīgōngnéng |
_version_ |
1719116100737695744 |