| Summary: | 碩士 === 國立中山大學 === 海洋科學系研究所 === 102 === The aim of the study was to investigate which of Vitamin E or oxidative status affect HUFA accumulation and synthesis in juvenile groupers. Two experiments were compared. In the experiment I, juvenile groupers were fed for 14 weeks one of the four HUFA-containing experimental diets: Diet FSE supplemented with both Vitamin E and Se, Diet FE supplemented with Vitamin E, Diet FS supplemented with Se, and Diet F supplemented with neither Vitamin E nor Se. The results of the growth trial showed no significant difference in growth performance. The F group had significantly lower crude lipids and HUFA contents than FS, FE, and FSE groups. Vitamin E supplementation resulted in a significantly increase in EPA accumulation. Its interaction with Se supplementation was significant. TBARS concentration in the liver were significantly affected by dietary Vitamin E and Se supplementation, highest in FS group and lowest in FSE group. There was no similar trend between the percentage of HUFA and concentration of TBARS in the liver. Fish fed Diet FE had the highest level of HUFA, but the FSE group that fed diet with both Vit. E and Se had the lowest TBARS concentration. In the experiment II, juvenile groupers were fed for 10 weeks, two HUFA-free experimental diets with (CS) or without (C) Se supplementation, to investigate which of biosynthesis or dietary residual affect the levels of HUFA by dietary antioxidant supplementation. Se supplementation resulted in a significant decrease in liver concentration of TBARS, but not in whole fish of HUFA levels. HUFA contents in the final fish of both C and CS groups whole body were significant lower than those of the initial fish. These results indicated Vitamin E, but not oxidative status, was the major factor affect if the level of HUFA in the grouper. When the diet was depleted in HUFA, the body HUFA levels in the groupers were derived from dietary residual, not from biosynthesis.
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