Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay
碩士 === 國立中山大學 === 海洋環境及工程學系研究所 === 102 === The first objective of this study was to investigate the removal efficiency of the endocrine disrupting chemicals(EDCs), including phenolic compounds such as nonylphenol(NP), octylphenol(OP) and bisphenol A(BPA), and natural and synthetic chemicals such as...
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ndltd-TW-102NSYS52820122019-05-15T21:32:36Z http://ndltd.ncl.edu.tw/handle/tv49ud Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay 利用生物試驗評估人工濕地雌激素活性之研究 Bo-Ya Yu 余博雅 碩士 國立中山大學 海洋環境及工程學系研究所 102 The first objective of this study was to investigate the removal efficiency of the endocrine disrupting chemicals(EDCs), including phenolic compounds such as nonylphenol(NP), octylphenol(OP) and bisphenol A(BPA), and natural and synthetic chemicals such as estrone(E1), 17β-estradiol(E2), estriol(E3), diethylstilbestrol(DES) and 17α-ethinylestradiol(EE2) of wastewater treated by the constructed wetland from the Wan-Dan embankment. The second objective was to assess the estrogenic activity with different treatment units using the T47D-KBluc bioassay. According to the T47D-KBluc assay, the estrogenicities in influent(In), grit chamber(GC), pre-aeration tank (AT), the contact aeration tank(CAT), aquatic plants area (PA) and effluent(Eff) were ranged from 1.18 to 30.4, 0.85 to 27.74, 0.46 to 3.32, 0.53 to 6.48, 0.48 to 13.44 and 0.52 to 21.16ng 17β-estradiol(E2) equivalents/L. The results of the different flows from the constructed wetland, 100CMD, 200CMD, 350CMD and 500CMD showed the mean removal efficiency of estrogenic activity, 64%, 42%, 36% and 31%, respectively. In chemical analysis, the samples were pre-concentrated by solid phase extraction, and analyzed for target compounds by LC-MS/MS method. According to the results, estrone, estradiol, ethynyl estradiol and diethylstilbestrol were not detected throughout the study period. The detection frequency of estriol, nonylphenol, octylphenol and bisphenol A were approximately 16%, 3%, 21% and 1%, respectively. The removal efficiency of estriol and octylphenol, showed was larger than 99% and 37%,respectively. Nonylphenol can’t be removed in the system. Bisphenol A detected only in one sample located in the aquatic plants area. Comparison of to the chemical analysis and bioassay, the concentration of estrogenic activity ranged from ND to 0.46ng / L and 0.48 to 30.3ng / L, respectively. Bioassay could be used to detect known and unknown estrogenic compounds, as a rapid and effective detection method. Lei Yang Chi-Ying Hsieh 楊磊 謝季吟 2014 學位論文 ; thesis 117 zh-TW |
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碩士 === 國立中山大學 === 海洋環境及工程學系研究所 === 102 === The first objective of this study was to investigate the removal efficiency of the endocrine disrupting chemicals(EDCs), including phenolic compounds such as nonylphenol(NP), octylphenol(OP) and bisphenol A(BPA), and natural and synthetic chemicals such as estrone(E1), 17β-estradiol(E2), estriol(E3), diethylstilbestrol(DES) and 17α-ethinylestradiol(EE2) of wastewater treated by the constructed wetland from the Wan-Dan embankment. The second objective was to assess the estrogenic activity with different treatment units using the T47D-KBluc bioassay.
According to the T47D-KBluc assay, the estrogenicities in influent(In), grit chamber(GC), pre-aeration tank (AT), the contact aeration tank(CAT), aquatic plants area (PA) and effluent(Eff) were ranged from 1.18 to 30.4, 0.85 to 27.74, 0.46 to 3.32, 0.53 to 6.48, 0.48 to 13.44 and 0.52 to 21.16ng 17β-estradiol(E2) equivalents/L. The results of the different flows from the constructed wetland, 100CMD, 200CMD, 350CMD and 500CMD showed the mean removal efficiency of estrogenic activity, 64%, 42%, 36% and 31%, respectively.
In chemical analysis, the samples were pre-concentrated by solid phase extraction, and analyzed for target compounds by LC-MS/MS method. According to the results, estrone, estradiol, ethynyl estradiol and diethylstilbestrol were not detected throughout the study period. The detection frequency of estriol, nonylphenol, octylphenol and bisphenol A were approximately 16%, 3%, 21% and 1%, respectively. The removal efficiency of estriol and octylphenol, showed was larger than 99% and 37%,respectively. Nonylphenol can’t be removed in the system. Bisphenol A detected only in one sample located in the aquatic plants area.
Comparison of to the chemical analysis and bioassay, the concentration of estrogenic activity ranged from ND to 0.46ng / L and 0.48 to 30.3ng / L, respectively. Bioassay could be used to detect known and unknown estrogenic compounds, as a rapid and effective detection method.
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author2 |
Lei Yang |
author_facet |
Lei Yang Bo-Ya Yu 余博雅 |
author |
Bo-Ya Yu 余博雅 |
spellingShingle |
Bo-Ya Yu 余博雅 Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay |
author_sort |
Bo-Ya Yu |
title |
Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay |
title_short |
Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay |
title_full |
Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay |
title_fullStr |
Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay |
title_full_unstemmed |
Assessment of Estrogenic Activity in a Constructed Wetland Using Bioassay |
title_sort |
assessment of estrogenic activity in a constructed wetland using bioassay |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/tv49ud |
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