Isolation of Farrow X-Y Spermatozoa on A Paper Chip Modified with Negative Micro Particles

• Main Author: 林育男
• Other Authors: 曾繁根
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/48h5ve

碩士 === 國立清華大學 === 工程與系統科學系 === 102 === As long as the blooming of livestock and high demand for dairy, livestock economy seems to turn into a hot direction. But the research and methods nowadays are not very value, so this paper wants to develop a method to differentiate the X and Y sperm of farrow. In that we can choose the offspring of livestock, which will become a huge property. Biochip is already become a mature way to select or detect not only protein but also germ and virus. So this paper wants to use the theory of biochip to differentiate the sperm in X and Y, but also wants to open up a new base-paper to make the chip. Traditional methods for differentiation efficiency like puresperm and albumin are not very powerful. Although the high efficiency ways like electrophoresis and flow cytometric will harm to the sperm. Because electrophoresis way will use electricity direct among the sperm so it will harm to them and flow cytometerichave to use the fluorescent dye. So these ways will make the survival rate of sperm be very low. In conclusion, this paper will focus on how to raise not only the differentiation efficiency but also the survival rate. This paper has the advantage of easy fabrication, just need printer and oven to make the chip on the filter paper. After the fabrication, spin the polystyrenebeads that modified with negative functional groups on the surface of the chip. At last, use plasma treatment on the chip. When run the experiment, just drop the sperm of farrow on the chip, and then wait for one minute to collect the liquid in the specific position. At last, use the flow cytometric to analysis. This research can achieve to separate the live sperm from dead and live sperm is in good quality. The differentiation efficiency can reach to 61:39 between X and Y sperm.