Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates
碩士 === 國立臺灣海洋大學 === 食品科學系 === 102 === The pulverized chlorella and freshwater clam were used as materials in this study. Pulverized chlorella were hydrolyzed by cellulase (Cellulase AP3) and protease (Protease N or Prozyme 6) at 50℃ for 1-5 hours. The pulverized Chlorella were hydrolyzed by Proteas...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2014
|
Online Access: | http://ndltd.ncl.edu.tw/handle/56867928573584302187 |
id |
ndltd-TW-102NTOU5253001 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-102NTOU52530012016-02-21T04:33:01Z http://ndltd.ncl.edu.tw/handle/56867928573584302187 Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates 綠藻與蜆肉水解物對血管收縮素轉換酶及二肽基胜肽酶 IV 活性之抑制 Wu Yen-Ching 吳彥慶 碩士 國立臺灣海洋大學 食品科學系 102 The pulverized chlorella and freshwater clam were used as materials in this study. Pulverized chlorella were hydrolyzed by cellulase (Cellulase AP3) and protease (Protease N or Prozyme 6) at 50℃ for 1-5 hours. The pulverized Chlorella were hydrolyzed by Protease N and Cellulase AP3, then fermented with lactic acid bacteria up to 40h to obtain chlorella Fermented hydrolysates. The freshwater clam were hydrolyzed by protease at 50℃ for 5 hours. The inhibitory effect of hydrolysates and Fermented hydrolysates on activity of DPP-IV (Dipepidy peptidase IV) were investigated. The pulverized chlorella were hydrolyzed by Cellulase AP3 and Protease N, their inhibition on DPP-IV with IC50 being 5.03 mg/mL, and it had higher inhibitory on DPP-IV with IC50 being 4.85-5.18 mg/mL in <10 KDa fraction. Chlorella Fermented hydrolysates on DPP-IV with IC50 were 7.17 mg/mL. Chlorella Fermented hydrolysates were separated into six fractions by size exclusion chromatography on a Sephadex G-25 gel with BPG column. The fraction FP5 (M.W.= 232-204 Da) of hydrolysate showed the better inhibition on DPP-IV with IC50 being 1.43 mg/ml.The fraction FP5 (M.W.= 232-204 Da) of hydrolysate showed the better inhibition on ACE (Angiotensin I converting enzyme) with IC50 being 28.7 μg/mL. Jenn-Shou Tsai 蔡震壽 2014 學位論文 ; thesis 45 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 國立臺灣海洋大學 === 食品科學系 === 102 === The pulverized chlorella and freshwater clam were used as materials in this study. Pulverized chlorella were hydrolyzed by cellulase (Cellulase AP3) and protease (Protease N or Prozyme 6) at 50℃ for 1-5 hours. The pulverized Chlorella were hydrolyzed by Protease N and Cellulase AP3, then fermented with lactic acid bacteria up to 40h to obtain chlorella Fermented hydrolysates. The freshwater clam were hydrolyzed by protease at 50℃ for 5 hours. The inhibitory effect of hydrolysates and Fermented hydrolysates on activity of DPP-IV (Dipepidy peptidase IV) were investigated. The pulverized chlorella were hydrolyzed by Cellulase AP3 and Protease N, their inhibition on DPP-IV with IC50 being 5.03 mg/mL, and it had higher inhibitory on DPP-IV with IC50 being 4.85-5.18 mg/mL in <10 KDa fraction. Chlorella Fermented hydrolysates on DPP-IV with IC50 were 7.17 mg/mL. Chlorella Fermented hydrolysates were separated into six fractions by size exclusion chromatography on a Sephadex G-25 gel with BPG column. The fraction FP5 (M.W.= 232-204 Da) of hydrolysate showed the better inhibition on DPP-IV with IC50 being 1.43 mg/ml.The fraction FP5 (M.W.= 232-204 Da) of hydrolysate showed the better inhibition on ACE (Angiotensin I converting enzyme) with IC50 being 28.7 μg/mL.
|
author2 |
Jenn-Shou Tsai |
author_facet |
Jenn-Shou Tsai Wu Yen-Ching 吳彥慶 |
author |
Wu Yen-Ching 吳彥慶 |
spellingShingle |
Wu Yen-Ching 吳彥慶 Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates |
author_sort |
Wu Yen-Ching |
title |
Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates |
title_short |
Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates |
title_full |
Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates |
title_fullStr |
Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates |
title_full_unstemmed |
Inhibition of Angiotensin I Converting Enzyme and Dipeptidyl Peptidase IV of Chlorella and Freshwater Clam Hydrolysates |
title_sort |
inhibition of angiotensin i converting enzyme and dipeptidyl peptidase iv of chlorella and freshwater clam hydrolysates |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/56867928573584302187 |
work_keys_str_mv |
AT wuyenching inhibitionofangiotensiniconvertingenzymeanddipeptidylpeptidaseivofchlorellaandfreshwaterclamhydrolysates AT wúyànqìng inhibitionofangiotensiniconvertingenzymeanddipeptidylpeptidaseivofchlorellaandfreshwaterclamhydrolysates AT wuyenching lǜzǎoyǔxiànròushuǐjiěwùduìxuèguǎnshōusuōsùzhuǎnhuànméijíèrtàijīshèngtàiméiivhuóxìngzhīyìzhì AT wúyànqìng lǜzǎoyǔxiànròushuǐjiěwùduìxuèguǎnshōusuōsùzhuǎnhuànméijíèrtàijīshèngtàiméiivhuóxìngzhīyìzhì |
_version_ |
1718194567944077312 |