Konjac Films Containing Au/Ag-Te Nanotubes as Antibacterial Wound Dressings

• Main Authors: Chang Nai-Wei, 張乃薇
• Other Authors: Yi-Cheng Huang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/gsv854

碩士 === 國立臺灣海洋大學 === 食品科學系 === 102 === Konjac glucomannan (KGM) had good swelling, water vapour transmission, non-cytotoxicity as well as provided wound healing on rat. However, it had not antibacterial property, so wound could not avoid infecting from bacterium. The aim of this study was to prepare KGM film which coupled into antibacterial agent, ‘gold/silver tellurium nanotube (Au/Ag-Te NT)’ for KGM film. We evaluated the Au/Ag-Te NT KGM film for antibacterial properties, cytotoxicity, blood clotting and wound healing. Au/Ag-Te NT produced Reactive oxygen species (ROS). The structure of Au/Ag-Te NT was rod shape composed by Ag/Te and a circle granule Au on the middle area, element analysis revealed that Au formed on the middle area. The X-ray diffraction (XRD) study revealed the major crystal facets for Au/Ag-Te NT at [101], [220] and [221]. The antibacterial test suggested when concentration of Au/Ag-Te NT KGM film higher than 3.40 μg/cm2 showed a good antibacterial property for Staphylococcus aureus and Pseudomonas aeruginosa, and in vitro test revealed that concentration lower than 3.40 μg/cm2 showed a good biocompatibility for L-929 mouse fibroblasts and HaCaT human keratinocyte, while cells attached Au/Ag-Te NT KGM film by pseudopodia. Au/Ag-Te NT KGM film also promoted blood clotting from clotting assay. In addition, Au/Ag-Te NT added did not influence physical properties of KGM film. In vivo test showed that Au/Ag-Te NT KGM film provided a significant higher percentage of wound closure compared with untreated control group and commercial dressing group in the 3rd , 7th and 10th day. Furthermore, histological examination demonstrated significantly advanced granulation tissue, angiogenesis and collagen formation in the 7th, 14th and 21st day. Histologic section revealed the granulation tissue and angiogenesis for Au/Ag-Te NT KGM film at early stage of wound healing in the 3rd and 5th day. These results suggested that Au/Ag-Te NT KGM film had good antibacterial property, biocompatibility, also promoted blood clotting and wound healing. It could be applied as a potential material of wound dressing.