The Preparation and Identification of Multifunctional Mesoporous Silica Nanoparticles for Dual-Mode Imaging, Theranostics, and Targeted Tracking in vivo

• Main Authors: Chan, Ming-Hsien, 詹明賢
• Other Authors: Lin, Hsiu-Mei
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/tb6359

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 102 === The use of nanoparticles imaging agents in diagnosis and therapy is receiving considerable attention in recent years, the research teams involving physicists and material chemists are interest in the multifunctional mesoporous silica nanoparticles (MSNs) because of the dual-model for imaging and therapy. This field is expanding rapidly, bringing a new term: theranostic, which means a combination of therapeutics and diagnostics. Comparing with the traditional imaging agent, MSNs have many advanced features to develop into a multifunctional platform by elaborate design. In order to improve the sensitivity of cellular examination and the effect of targeting, the multifunctional MSNs are going to combine dual imaging, cellular control and folate-modified to contain the ability of therapy and diagnosis to apply for theranostic biological field. This complex multifunctional MSNs as an imaging and therapeutic agent can accumulate a sufficient amount at lesion, with great prospects of theranostic for the future. This research conducts with three parts: first, doping Eu3+ and Gd3+ into the MSNs to synthesize the MSN-EuGd with the fluorescence and magnesium dual imaging function; secondly, conjugating the intracellular-controlled drug on MSN; finally, grafting the folate molecular on MSN-EuGd/drug. According to our research experience, this multifunctional nano-platform could bring the dual imaging, cellular control and folate together by optimal conditions to prepare the multifunctional MSN-EuGd/drug/folate. Furthermore, the physical and chemical properties of EuGd-MSN/drug/folate were investigated to test in vitro and in vivo assessment.