| Summary: | 碩士 === 國立臺灣大學 === 森林環境暨資源學研究所 === 102 === The main objectives of this study are to purpose and establish the micropropagation and somatic embryogenesis system in the tissue culture of Pongamia pinnata.
Results of nodal stem segments of epicormics culture of P. pinnata were as followed:nodal stem segments were first treated with running water for two hr. , shaking in 1/300 (v/v) anticeptol solution (Benzel thyonium chloride U.S.P 10﹪ (w/v) , Alkyl aryl polyether alcohol 10﹪(w/v)） for 30 sec , then shaking in 70﹪ethanol solution for 30 seconds, then soaked in 5﹪NaOCl (supplemented with 0.1﹪ (v/v) Tween 20） and treated with ultrasonic shaking for 30 min and the nodal stem segments obtained 65﹪non contamination.
In the aspect of organ formation : the medium of 1 mg/L BA in MS or WPM gained the best results of aseptic seedlings. Micropropagation of P. pinnata from cotyledonary nodes, nodal stem, epicotyls and hypocotyls explants derived from in vitro seedlings. Multiple shoots induction from cotyledonary node explants gained the best result of that from other explants. The greatest shoots were induced from the 1 mg/L BA pretreatmenting of cotyledonary nodes explants was achieved when MS was added with 2 mg/L BA and 0.1 mg/L NAA, average of 5.42 shoots per explants were formed in one month. Spring buds at the stage of ready to burst from dormancy and shoot explants in spring were collected from the coppice shoots of P. pinnata wild trees. MS medium containing 1-2 mg/L BA and 0.1 mg/L NAA was the best for inducing more shoots. Shoot elongation of these multiple shoots was good at 1 mg/L GA3 and 0.2 mg/L BA in MS medium. Individual shoot were rooted on 1/2MS or MS supplemented with IBA. The 1 mg/L IBA in 1/2MS medium gave the highest percentage of rooting (74.55%) with root number of 2.96 roots/shoot and root length of 1.33 cm. Cotyledons were induced with the highest percentage of 20.83% and with average of 3 somatic embryo/per explant of somatic embryogenesis in media composed of MS medium supplemented with 2,4-D 3 mg/L and Kn 0.5 mg/L after two months.
Shoots with roots were suitable for acclimatization. The plantlets survival rates were 85.88% with average shoot length of 0.78 cm after six weeks of acclimatization.
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